Virulence Profiles and Genome-Wide Association Study for Ascochyta lentis Isolates Collected from Australian Lentil-Growing Regions.

Autor:	Henares BM; Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA 6102, Australia., Blake SN; Pulse and Oilseed Pathology, Plant Health & Biosecurity, Crop Sciences, South Australian Research and Development Institute, Adelaide, SA 5064 Australia., Farfan-Caceres L; Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA 6102, Australia., Tahghighi H; Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA 6102, Australia., Debler JW; Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA 6102, Australia., Russ MH; Pulse and Oilseed Pathology, Plant Health & Biosecurity, Crop Sciences, South Australian Research and Development Institute, Adelaide, SA 5064 Australia., Farquharson EA; Pulse and Oilseed Pathology, Plant Health & Biosecurity, Crop Sciences, South Australian Research and Development Institute, Adelaide, SA 5064 Australia., Rose JA; Pulse and Oilseed Pathology, Plant Health & Biosecurity, Crop Sciences, South Australian Research and Development Institute, Adelaide, SA 5064 Australia., Khani M; Pulse and Oilseed Pathology, Plant Health & Biosecurity, Crop Sciences, South Australian Research and Development Institute, Adelaide, SA 5064 Australia., Davidson JA; Pulse and Oilseed Pathology, Plant Health & Biosecurity, Crop Sciences, South Australian Research and Development Institute, Adelaide, SA 5064 Australia., Kamphuis LG; Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA 6102, Australia., Lee RC; Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA 6102, Australia.
Jazyk:	angličtina
Zdroj:	Phytopathology [Phytopathology] 2023 Aug; Vol. 113 (8), pp. 1515-1524. Date of Electronic Publication: 2023 Oct 01.
DOI:	10.1094/PHYTO-10-22-0397-R
Abstrakt:	Ascochyta lentis , the causal organism of Ascochyta blight (AB) of lentil ( Lens culinaris ), has been shown to produce an avirulence effector protein that mediates AB resistance in certain lentil cultivars. The two known forms of the effector protein were identified from a biparental mapping population between isolates that have reciprocal virulence on 'PBA Hurricane XT' and 'Nipper'. The effector AlAvr1-1 was described for the PBA Hurricane XT-avirulent isolate P94-24 and AlAvr1-2 characterized in the PBA Hurricane XT-virulent isolate Al Kewell. Here, we performed a genome-wide association study to identify other loci associated with AB for a differential set of lentil cultivars from a diverse panel of isolates collected in the Australian lentil-growing regions from 2013 to 2020. The chromosome 3 AlAvr1 locus was strongly associated with the PBA Hurricane XT, 'Indianhead', and Nipper disease responses, but one other genomic region on chromosome 11 was also associated with the Nipper disease trait. Our results corroborate earlier work that identified the AlAvr1 locus for field-collected isolates that span the period before release and after widespread adoption of PBA Hurricane XT. A multiplex PCR assay was developed to differentiate the genes AlAvr1-1 and AlAvr1-2 to predict PBA Hurricane XT avirulence and pathotype designation in the diversity panel. Increasing numbers of the PBA Hurricane XT-virulent pathotype 2 isolates across that time indicate strong selection for isolates with the AlAvr1-2 allele. Furthermore, one other region of the A. lentis genome may contribute to the pathogen-host interaction for lentil AB. Competing Interests: The author(s) declare no conflict of interest.
Databáze:	MEDLINE
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