SARS-CoV-2 spike-ferritin-nanoparticle adjuvanted with ALFQ induces long-lived plasma cells and cross-neutralizing antibodies.

Autor: Shrivastava S; Laboratory of Adjuvant and Antigen Research, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Carmen JM; Laboratory of Adjuvant and Antigen Research, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Center for Infectious Disease Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Lu Z; Department of Pediatrics, F. Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD, USA., Basu S; Laboratory of Adjuvant and Antigen Research, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Sankhala RS; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Chen WH; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Nguyen P; Laboratory of Adjuvant and Antigen Research, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Chang WC; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA., King J; Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Corbitt C; Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Mayer S; Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Bolton JS; Malaria Biologics Branch, Walter Reed Army Institute of Research, Silver Spring, USA., Anderson A; Laboratory of Adjuvant and Antigen Research, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Oak Ridge Institute of Science and Education, Oak Ridge, TN, 37831, USA., Swafford I; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Terriquez GD; Laboratory of Adjuvant and Antigen Research, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Trinh HV; Laboratory of Adjuvant and Antigen Research, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Kim J; Laboratory of Adjuvant and Antigen Research, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Jobe O; Laboratory of Adjuvant and Antigen Research, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Paquin-Proulx D; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Matyas GR; Laboratory of Adjuvant and Antigen Research, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Center for Infectious Disease Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Gromowski GD; Center for Infectious Disease Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Currier JR; Center for Infectious Disease Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Bergmann-Leitner E; Center for Infectious Disease Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Malaria Biologics Branch, Walter Reed Army Institute of Research, Silver Spring, USA., Modjarrad K; Center for Infectious Disease Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Michael NL; Center for Infectious Disease Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Joyce MG; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Malloy AMW; Department of Pediatrics, F. Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD, USA., Rao M; Laboratory of Adjuvant and Antigen Research, U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA. mrao@hivresearch.org.; Center for Infectious Disease Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA. mrao@hivresearch.org.
Jazyk: angličtina
Zdroj: NPJ vaccines [NPJ Vaccines] 2023 Mar 18; Vol. 8 (1), pp. 43. Date of Electronic Publication: 2023 Mar 18.
DOI: 10.1038/s41541-023-00638-6
Abstrakt: This study demonstrates the impact of adjuvant on the development of T follicular helper (Tfh) and B cells, and their influence on antibody responses in mice vaccinated with SARS-CoV-2-spike-ferritin-nanoparticle (SpFN) adjuvanted with either Army Liposome Formulation containing QS-21 (SpFN + ALFQ) or Alhydrogel ® (SpFN + AH). SpFN + ALFQ increased the size and frequency of germinal center (GC) B cells in the vaccine-draining lymph nodes and increased the frequency of antigen-specific naive B cells. A single vaccination with SpFN + ALFQ resulted in a higher frequency of IL-21-producing-spike-specific Tfh and GC B cells in the draining lymph nodes and spleen, S-2P protein-specific IgM and IgG antibodies, and elicitation of robust cross-neutralizing antibodies against SARS-CoV-2 variants as early as day 7, which was enhanced by a second vaccination. This was associated with the generation of high titer, high avidity binding antibodies. The third vaccination with SpFN + ALFQ elicited high levels of neutralizing antibodies against the Omicron variant. No cross-neutralizing antibodies against Omicron were induced with SpFN + AH. These findings highlight the importance of ALFQ in orchestrating early induction of antigen-specific Tfh and GC B cell responses and long-lived plasma cells in the bone marrow. The early engagement of S-2P specific naive B cells and high titer IgM antibodies shape the development of long-term neutralization breadth.
(© 2023. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
Databáze: MEDLINE
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