Over-expression of the barley Phytoglobin 1 (HvPgb1) evokes leaf-specific transcriptional responses during root waterlogging.

Autor: Hill RD; Department of Plant Science, University of Manitoba, Winnipeg, R3T2N2, MB, Canada., de Castro J; Department of Plant Science, University of Manitoba, Winnipeg, R3T2N2, MB, Canada., Mira MM; Department of Plant Science, University of Manitoba, Winnipeg, R3T2N2, MB, Canada; Department of Botany and Microbiology, Tanta University, Tanta, Egypt. Electronic address: mohammed.mira@umanitoba.ca., Igamberdiev AU; Department of Biology, Memorial University of Newfoundland, St. John's, NL, A1C5S7, Canada., Hebelstrup KH; Department of Molecular Biology and Genetics, University of Aarhus, Forsogsvej 1, 4200, Slagelse, Denmark., Renault S; Department of Biological Sciences, University of Manitoba, Winnipeg, R3T2N2, MB, Canada., Xu W; Brandon Research and Development Center, Agriculture and Agri-Food Canada, 2701 Grand Valley Road, Brandon, MB, R7A 5Y3, Canada., Badea A; Brandon Research and Development Center, Agriculture and Agri-Food Canada, 2701 Grand Valley Road, Brandon, MB, R7A 5Y3, Canada., Stasolla C; Department of Plant Science, University of Manitoba, Winnipeg, R3T2N2, MB, Canada. Electronic address: Claudio.stasolla@umanitoba.ca.
Jazyk: angličtina
Zdroj: Journal of plant physiology [J Plant Physiol] 2023 Apr; Vol. 283, pp. 153944. Date of Electronic Publication: 2023 Feb 25.
DOI: 10.1016/j.jplph.2023.153944
Abstrakt: Oxygen deprivation (hypoxia) in the root due to waterlogging causes profound metabolic changes in the aerial organs depressing growth and limiting plant productivity in barley (Hordeum vulgare L.). Genome-wide analyses in waterlogged wild type (WT) barley (cv. Golden Promise) plants and plants over-expressing the phytoglobin 1 HvPgb1 [HvPgb1(OE)] were performed to determine leaf specific transcriptional responses during waterlogging. Normoxic WT plants outperformed their HvPgb1(OE) counterparts for dry weight biomass, chlorophyll content, photosynthetic rate, stomatal conductance, and transpiration. Root waterlogging severely depressed all these parameters in WT plants but not in HvPgb1(OE) plants, which exhibited an increase in photosynthetic rate. In leaftissue, root waterlogging repressed genes encoding photosynthetic components and chlorophyll biosynthetic enzymes, while induced those of reactive oxygen species (ROS)-generating enzymes. This repression was alleviated in HvPgb1(OE) leaves which also exhibited an induction of enzymes participating in antioxidant responses. In the same leaves, the transcript levels of several genes participating in nitrogen metabolism were also higher relative to WT leaves. Ethylene levels were diminished by root waterlogging in leaves of WT plants, but not in HvPgb1(OE), which were enriched in transcripts of ethylene biosynthetic enzymes and ethylene response factors. Pharmacological treatments increasing the level or action of ethylene further suggested the requirement of ethylene in plant response to root waterlogging. In natural germplasm an elevation in foliar HvPgb1 between 16h and 24h of waterlogging occurred in tolerant genotypes but not in susceptible ones. By integrating morpho-physiological parameters with transcriptome data, this study provides a framework defining leaf responses to root waterlogging and indicates that the induction of HvPgb1 may be used as a selection tool to enhance resilience to excess moisture.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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