The extracellular matrix protein pattern in the canine neoplastic mammary gland.

Autor: Barreto RSN; Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270 Butantã, São Paulo, Brazil., Carvalho HJC; Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270 Butantã, São Paulo, Brazil., Matias GSS; Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270 Butantã, São Paulo, Brazil., Silva MGKC; Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270 Butantã, São Paulo, Brazil., Ribeiro RR; Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270 Butantã, São Paulo, Brazil., Campanelli TB; Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270 Butantã, São Paulo, Brazil., Rigoglio NN; Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270 Butantã, São Paulo, Brazil., Carreira ACO; Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270 Butantã, São Paulo, Brazil; Center for Natural and Human Sciences, Federal University of ABC, Av. dos Estados, 5001, Bairro Santa Terezinha, 09210-580 Santo André, Brazil., Miglino MA; Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270 Butantã, São Paulo, Brazil. Electronic address: miglino@usp.br.
Jazyk: angličtina
Zdroj: Tissue & cell [Tissue Cell] 2023 Jun; Vol. 82, pp. 102050. Date of Electronic Publication: 2023 Feb 24.
DOI: 10.1016/j.tice.2023.102050
Abstrakt: Extracellular matrix (ECM) proteins in the mammary gland provide structure and regulate its development and homeostasis. Alterations in its structure can regulate and support pathogenesis, like breast tumors. Aiming to identify the health and tumoral canine mammary ECM scaffold protein profile by immunohistochemistry, the decellularization process was carried out to remove the cellular content. Additionally, it was verified the influence of health and tumoral ECM on the attachment of health and tumoral cells. The types I, III, IV, and V structural collagens were scarce in the mammary tumor, and ECM fibers were disorganized. Vimentin and CD44 were more common in mammary tumor stroma, suggesting a role in cell migration that results in tumor progression. Elastin, fibronectin, laminin, vitronectin, and osteopontin were similarly detected under healthy and tumor conditions, providing the attachment of normal cells in healthy ECM, while tumoral cells were able to attach in tumoral ECM. The protein pattern demonstrates ECM alteration in canine mammary tumorigenesis, presenting new knowledge on mammary tumor ECM microenvironment.
Competing Interests: Conflict of interest The authors declare that the research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023. Published by Elsevier Ltd.)
Databáze: MEDLINE
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