Heterologous Assembly of Pleomorphic Bacterial Microcompartment Shell Architectures Spanning the Nano- to Microscale.

Autor: Ferlez BH; MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI, 48824, USA.; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48824, USA., Kirst H; MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI, 48824, USA.; Environmental Genomics and Systems Biology and Molecular Biophysics and Integrative Bioimaging Divisions, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Greber BJ; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, Berkeley, CA, 94720, USA.; Molecular Biophysics and Integrative Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Nogales E; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, Berkeley, CA, 94720, USA.; Molecular Biophysics and Integrative Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA, 94720, USA.; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA., Sutter M; MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI, 48824, USA.; Environmental Genomics and Systems Biology and Molecular Biophysics and Integrative Bioimaging Divisions, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Kerfeld CA; MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI, 48824, USA.; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48824, USA.; Environmental Genomics and Systems Biology and Molecular Biophysics and Integrative Bioimaging Divisions, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Jazyk: angličtina
Zdroj: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2023 Jun; Vol. 35 (23), pp. e2212065. Date of Electronic Publication: 2023 Apr 25.
DOI: 10.1002/adma.202212065
Abstrakt: Many bacteria use protein-based organelles known as bacterial microcompartments (BMCs) to organize and sequester sequential enzymatic reactions. Regardless of their specialized metabolic function, all BMCs are delimited by a shell made of multiple structurally redundant, yet functionally diverse, hexameric (BMC-H), pseudohexameric/trimeric (BMC-T), or pentameric (BMC-P) shell protein paralogs. When expressed without their native cargo, shell proteins have been shown to self-assemble into 2D sheets, open-ended nanotubes, and closed shells of ≈40 nm diameter that are being developed as scaffolds and nanocontainers for applications in biotechnology. Here, by leveraging a strategy for affinity-based purification, it is demonstrated that a wide range of empty synthetic shells, many differing in end-cap structures, can be derived from a glycyl radical enzyme-associated microcompartment. The range of pleomorphic shells observed, which span ≈2 orders of magnitude in size from ≈25 nm to ≈1.8 µm, reveal the remarkable plasticity of BMC-based biomaterials. In addition, new capped nanotube and nanocone morphologies are observed that are consistent with a multicomponent geometric model in which architectural principles are shared among asymmetric carbon, viral protein, and BMC-based structures.
(© 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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