miR-322 promotes the differentiation of embryonic stem cells into cardiomyocytes.

Autor: Liu K; Department of Cardiovascular, Ganzhou People's Hospital, Jiangxi, China. liu593008816@163.com.; Ganzhou, 341000, Jiangxi, China. liu593008816@163.com., Peng X; Department of Cardiovascular, the First Affiliated Hospital of Nanchang University, Nanchang, China., Luo L; Department of Cardiovascular, Ganzhou People's Hospital, Jiangxi, China.
Jazyk: angličtina
Zdroj: Functional & integrative genomics [Funct Integr Genomics] 2023 Mar 18; Vol. 23 (2), pp. 87. Date of Electronic Publication: 2023 Mar 18.
DOI: 10.1007/s10142-023-01008-0
Abstrakt: Previous studies have shown that miR-322 regulates the functions of various stem cells. However, the role and mechanism of embryonic stem cell (ESCs) differentiation into cardiomyocytes remains unknown. Celf1 plays a vital role in stem cell differentiation and may be a potential target of miR-322 in ESCs' differentiation. We studied the function of miR-322An using mESCs transfected with lentivirus-mediated miR-322. RT-PCR results indicated that miR-322 increased NKX-2.5, MLC2V, and α-MHC mRNA expression, signifying that miR-322 might promote the differentiation of ESCs toward cardiomyocytes in vitro. The western blotting and immunofluorescence results confirmed this conclusion. In addition, the knockdown of miR-322 expression inhibited ESCs' differentiation toward cardiomyocytes in cultured ESCs in vitro. Western blotting results showed that miR-322 suppressed celf1 protein expression. Furthermore, Western blotting, RT-PCR, and immunofluorescence results showed that celf1 may inhibit ESCs' differentiation toward cardiomyocytes in vitro. Overall, the results indicate that miR-322 might promote ESCs' differentiation toward cardiomyocytes by regulating celf1 expression.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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