Coliphage cocktails for controlling antimicrobial-resistant Escherichia coli: emphasizing polyphage and multihost interactions at different levels of multiplicity of infection.

Autor:	Benala M; Visakhapatnam Research Centre of ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Visakhapatnam 530003, India.; Department of Microbiology and FST, School of Science, GITAM, Visakhapatnam 530045, India., Vaiyapuri M; ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Willingdon Island, Cochin 682029, India., Raveendran K; ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Willingdon Island, Cochin 682029, India., George JC; ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Willingdon Island, Cochin 682029, India., Sivam V; ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Willingdon Island, Cochin 682029, India., Badireddy MR; Visakhapatnam Research Centre of ICAR-Central Institute of Fisheries Technology (ICAR-CIFT), Visakhapatnam 530003, India.
Jazyk:	angličtina
Zdroj:	Journal of applied microbiology [J Appl Microbiol] 2023 Apr 03; Vol. 134 (4).
DOI:	10.1093/jambio/lxad055
Abstrakt:	Aim: This study elucidates the in-vitro bactericidal effectiveness of polyphage cocktail combinations of 2, 4, 6, 8, and 10 individual coliphages against a cocktail of 20 AMR Escherichia coli. Methods and Results: Different polyphage cocktails viz., 45 two-phage combinations, 28 four-phage combinations, 15 six-phage combinations, 6 eight-phage combinations, and 1 ten-phage combination were formulated using a pool of ten coliphages that were isolated from two different geographical locations (East and West coasts of India). The different polyphage cocktails were tested at four different levels of Multiplicity of Infection (MOI) viz., MOI-1, MOI-10, MOI-100, and MOI-1000. All the 2, 4, 6, 8, and 10-phage cocktails were found to be effective in controlling the growth of a cocktail of 20 AMR bacteria when tested at MOI-1000 and MOI-100 but variations in antibacterial activity were observed at lower MOIs of 10 and 1. The ten coliphage cocktail showed lytic activity against 100% of AMR E. coli from farmed brackish water shrimp, 96% of laboratory collection of AMR E. coli, 92% of AMR E. coli from farmed freshwater fish, and 85% of AMR E. coli from market shrimp. Conclusion: Polyphage cocktails of 2, 4, 6, 8, and 10 coliphages applied at an MOI of 1000 effectively suppressed the growth of antimicrobial-resistant E. coli. The results indicated phage-phage synergy in the lytic activity of several coliphage combinations at higher MOIs of 1000 and 100 while phage-phage antagonism was evidenced at lower MOIs of 10 and 1. (© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
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