Preparation of pumpkin oil-based nanoemulsion as a potential estrogen replacement therapy to alleviate neural-immune interactions in an experimental postmenopausal model.

Autor: El-Bana MA; Medical Biochemistry Department, Medical Research and Clinical Studies Institute National Research Centre, Dokki, Giza, Egypt., El-Daly SM; Medical Biochemistry Department, Medical Research and Clinical Studies Institute National Research Centre, Dokki, Giza, Egypt; Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Dokki, Giza, Egypt., Omara EA; Pathology Department, Medical Research and Clinical Studies Institute, National Research Centre, Dokki, Giza, Egypt., Morsy SM; Medical Biochemistry Department, Medical Research and Clinical Studies Institute National Research Centre, Dokki, Giza, Egypt., El-Naggar ME; Institute of Textile Research and Technology, National Research Centre, Dokki, Giza, Egypt., Medhat D; Medical Biochemistry Department, Medical Research and Clinical Studies Institute National Research Centre, Dokki, Giza, Egypt. Electronic address: dalia_8383@hotmail.com.
Jazyk: angličtina
Zdroj: Prostaglandins & other lipid mediators [Prostaglandins Other Lipid Mediat] 2023 Jun; Vol. 166, pp. 106730. Date of Electronic Publication: 2023 Mar 15.
DOI: 10.1016/j.prostaglandins.2023.106730
Abstrakt: As estrogen production decreases during menopause; the brain's metabolism tends to stall and become less effective. Estrogen most likely protects against neurodegeneration. Consequently, a comprehensive study of the benefits of hormone replacement therapy as a neuroprotective effect is urgently required. This study was designed to fabricate pumpkin seed oil nanoparticles (PSO) in nanoemulsion form (PSO-NE) and investigate their potential role in attenuating the neural-immune interactions in an experimental postmenopausal model.Sixty female white albino rats were divided into six groups: control, sham, ovariectomized (OVX), and three OVX groups treated with 17β-estradiol, PSO, and PSO-NE respectively. Transmission Electron Microscopy (TEM), and particle size analyzer were performed for nanoemulsion evaluation. Serum levels of estrogen, brain amyloid precursor protein (APP), serum levels of nuclear factor kappa B (NF-κβ), interleukin 6 (IL-6), transthyretin (TTR), and synaptophysin (SYP) were evaluated. The expression of estrogen receptors (ER-α, β) in the brain tissue was estimated. The findings revealed that the approached PSO-NE system was able to reduce the interfacial tension, enhance the dispersion entropy, lower the system free energy to an extremely small value, and augment the interfacial area. PSO-NE, showed a significant increase in the levels of estrogen, brain APP, SYP, and TTR accompanied with a significant increased in the expression of brain ER-α, β compared to the OVX group. In conclusion, the phytoestrogen content of PSO exhibited a significant prophylactic effect on neuro-inflammatory interactions, ameliorating both estrogen levels and the inflammatory cascades.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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