Plant and prokaryotic TIR domains generate distinct cyclic ADPR NADase products.

Autor:	Bayless AM; Department of Biology, Colorado State University, Fort Collins, CO 80523, USA., Chen S; National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China., Ogden SC; Department of Biology, Colorado State University, Fort Collins, CO 80523, USA.; Cell and Molecular Biology Graduate Program, Colorado State University, Fort Collins, CO 80523, USA., Xu X; National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China., Sidda JD; School of Life Sciences, University of Warwick, Coventry CV47AL, UK., Manik MK; The University of Queensland, School of Chemistry and Molecular Biosciences, Australian Infectious Diseases Research Centre and Institute for Molecular Bioscience, Brisbane, QLD 4072, Australia., Li S; The University of Queensland, School of Chemistry and Molecular Biosciences, Australian Infectious Diseases Research Centre and Institute for Molecular Bioscience, Brisbane, QLD 4072, Australia., Kobe B; The University of Queensland, School of Chemistry and Molecular Biosciences, Australian Infectious Diseases Research Centre and Institute for Molecular Bioscience, Brisbane, QLD 4072, Australia., Ve T; Institute for Glycomics, Griffith University, Southport, QLD 4222, Australia., Song L; School of Life Sciences, University of Warwick, Coventry CV47AL, UK., Grant M; School of Life Sciences, University of Warwick, Coventry CV47AL, UK., Wan L; National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China., Nishimura MT; Department of Biology, Colorado State University, Fort Collins, CO 80523, USA.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2023 Mar 15; Vol. 9 (11), pp. eade8487. Date of Electronic Publication: 2023 Mar 17.
DOI:	10.1126/sciadv.ade8487
Abstrakt:	Toll/interleukin-1 receptor (TIR) domain proteins function in cell death and immunity. In plants and bacteria, TIR domains are often enzymes that produce isomers of cyclic adenosine 5'-diphosphate-ribose (cADPR) as putative immune signaling molecules. The identity and functional conservation of cADPR isomer signals is unclear. A previous report found that a plant TIR could cross-activate the prokaryotic Thoeris TIR-immune system, suggesting the conservation of plant and prokaryotic TIR-immune signals. Here, we generate autoactive Thoeris TIRs and test the converse hypothesis: Do prokaryotic Thoeris TIRs also cross-activate plant TIR immunity? Using in planta and in vitro assays, we find that Thoeris and plant TIRs generate overlapping sets of cADPR isomers and further clarify how plant and Thoeris TIRs activate the Thoeris system via producing 3'cADPR. This study demonstrates that the TIR signaling requirements for plant and prokaryotic immune systems are distinct and that TIRs across kingdoms generate a diversity of small-molecule products.
Databáze:	MEDLINE
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