Bacteria require phase separation for fitness in the mammalian gut.

Autor:	Krypotou E; Department of Microbial Pathogenesis, Yale School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.; Yale Microbial Sciences Institute, P.O. Box 27389, West Haven, CT 06516, USA., Townsend GE; Department of Microbial Pathogenesis, Yale School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.; Yale Microbial Sciences Institute, P.O. Box 27389, West Haven, CT 06516, USA.; Department of Biochemistry and Molecular Biology, Penn State College of Medicine, 700 HMC Crescent Road, Hershey, PA 17033, USA., Gao X; Department of Microbial Pathogenesis, Yale School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA., Tachiyama S; Department of Microbial Pathogenesis, Yale School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.; Yale Microbial Sciences Institute, P.O. Box 27389, West Haven, CT 06516, USA., Liu J; Department of Microbial Pathogenesis, Yale School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.; Yale Microbial Sciences Institute, P.O. Box 27389, West Haven, CT 06516, USA., Pokorzynski ND; Department of Microbial Pathogenesis, Yale School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA., Goodman AL; Department of Microbial Pathogenesis, Yale School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.; Yale Microbial Sciences Institute, P.O. Box 27389, West Haven, CT 06516, USA., Groisman EA; Department of Microbial Pathogenesis, Yale School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.; Yale Microbial Sciences Institute, P.O. Box 27389, West Haven, CT 06516, USA.
Jazyk:	angličtina
Zdroj:	Science (New York, N.Y.) [Science] 2023 Mar 17; Vol. 379 (6637), pp. 1149-1156. Date of Electronic Publication: 2023 Mar 16.
DOI:	10.1126/science.abn7229
Abstrakt:	Therapeutic manipulation of the gut microbiota holds great potential for human health. The mechanisms bacteria use to colonize the gut therefore present valuable targets for clinical intervention. We now report that bacteria use phase separation to enhance fitness in the mammalian gut. We establish that the intrinsically disordered region (IDR) of the broadly and highly conserved transcription termination factor Rho is necessary and sufficient for phase separation in vivo and in vitro in the human commensal Bacteroides thetaiotaomicron . Phase separation increases transcription termination by Rho in an IDR-dependent manner. Moreover, the IDR is critical for gene regulation in the gut. Our findings expose phase separation as vital for host-commensal bacteria interactions and relevant for novel clinical applications.
Databáze:	MEDLINE
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