Computer-aided classification of indirect immunofluorescence patterns on esophagus and split skin for the detection of autoimmune dermatoses.
Autor: | Hocke J; Institute for Experimental Immunology, affiliated to EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany., Krauth J; Institute for Experimental Immunology, affiliated to EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany., Krause C; Institute for Experimental Immunology, affiliated to EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany., Gerlach S; Institute for Experimental Immunology, affiliated to EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany., Warnemünde N; Institute for Experimental Immunology, affiliated to EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany., Affeldt K; Institute for Experimental Immunology, affiliated to EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany., van Beek N; Department of Dermatology, Allergology and Venerology, University Hospital Schleswig-Holstein/University of Lübeck, Lübeck, Germany., Schmidt E; Department of Dermatology, Allergology and Venerology, University Hospital Schleswig-Holstein/University of Lübeck, Lübeck, Germany.; Lübeck Institute of Experimental Dermatology (LIED), University of Lübeck, Lübeck, Germany., Voigt J; Institute for Experimental Immunology, affiliated to EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany. |
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Jazyk: | angličtina |
Zdroj: | Frontiers in immunology [Front Immunol] 2023 Feb 28; Vol. 14, pp. 1111172. Date of Electronic Publication: 2023 Feb 28 (Print Publication: 2023). |
DOI: | 10.3389/fimmu.2023.1111172 |
Abstrakt: | Autoimmune bullous dermatoses (AIBD) are rare diseases that affect human skin and mucous membranes. Clinically, they are characterized by blister formation and/or erosions. Depending on the structures involved and the depth of blister formation, they are grouped into pemphigus diseases, pemphigoid diseases, and dermatitis herpetiformis. Classification of AIBD into their sub-entities is crucial to guide treatment decisions. One of the most sensitive screening methods for initial differentiation of AIBD is the indirect immunofluorescence (IIF) microscopy on tissue sections of monkey esophagus and primate salt-split skin, which are used to detect disease-specific autoantibodies. Interpretation of IIF patterns requires a detailed examination of the image by trained professionals automating this process is a challenging task with these highly complex tissue substrates, but offers the great advantage of an objective result. Here, we present computer-aided classification of esophagus and salt-split skin IIF images. We show how deep networks can be adapted to the specifics and challenges of IIF image analysis by incorporating segmentation of relevant regions into the prediction process, and demonstrate their high accuracy. Using this semi-automatic extension can reduce the workload of professionals when reading tissue sections in IIF testing. Furthermore, these results on highly complex tissue sections show that further integration of semi-automated workflows into the daily workflow of diagnostic laboratories is promising. Competing Interests: JV, JH, SG, JK, CK, NW, and KA are employees of EUROIMMUN Medizinische Labordiagnostika AG. ES has a scientific cooperation with EUROIMMUN. JV, JH, SG, JK, CK, MH, ES, and NB are inventors of the patent application number 22199236 that has been filed by EUROIMMUN Medizinische Labordiagnostika AG on 30th Sept 2022. This patent application is pending and covers the algorithmic processing of the salt-split skin substrate. (Copyright © 2023 Hocke, Krauth, Krause, Gerlach, Warnemünde, Affeldt, van Beek, Schmidt and Voigt.) |
Databáze: | MEDLINE |
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