Temporal evaluation of efficacy and quality of tissue repair upon laser-activated sealing.

Autor: Ghosh D; Biological Design Graduate Program, School for Engineering of Matter, Transport, and Energy Arizona State University Tempe Arizona USA., Salinas CM; James C. Wyant College of Optical Sciences University of Arizona Tucson Arizona USA., Pallod S; Biological Design Graduate Program, School for Engineering of Matter, Transport, and Energy Arizona State University Tempe Arizona USA., Roberts J; School of Life Sciences Arizona State University Tempe Arizona USA., Makin IRS; School of Osteopathic Medicine A.T. Still University Mesa Arizona USA., Yaron JR; Biological Design Graduate Program, School for Engineering of Matter, Transport, and Energy Arizona State University Tempe Arizona USA.; Department of Chemical Engineering, School for Engineering of Matter, Transport, and Energy Arizona State University Tempe Arizona USA., Witte RS; James C. Wyant College of Optical Sciences University of Arizona Tucson Arizona USA.; Department of Medical Imaging University of Arizona Tucson Arizona USA., Rege K; Biological Design Graduate Program, School for Engineering of Matter, Transport, and Energy Arizona State University Tempe Arizona USA.; Department of Chemical Engineering, School for Engineering of Matter, Transport, and Energy Arizona State University Tempe Arizona USA.
Jazyk: angličtina
Zdroj: Bioengineering & translational medicine [Bioeng Transl Med] 2022 Sep 28; Vol. 8 (2), pp. e10412. Date of Electronic Publication: 2022 Sep 28 (Print Publication: 2023).
DOI: 10.1002/btm2.10412
Abstrakt: Injuries caused by surgical incisions or traumatic lacerations compromise the structural and functional integrity of skin. Immediate approximation and robust repair of skin are critical to minimize occurrences of dehiscence and infection that can lead to impaired healing and further complication. Light-activated skin sealing has emerged as an alternative to sutures, staples, and superficial adhesives, which do not integrate with tissues and are prone to scarring and infection. Here, we evaluate both shorter- and longer-term efficacy of tissue repair response following laser-activated sealing of full-thickness skin incisions in immunocompetent mice and compare them to the efficacy seen with sutures. Laser-activated sealants (LASEs) in which, indocyanine green was embedded within silk fibroin films, were used to form viscous pastes and applied over wound edges. A hand-held, near-infrared laser was applied over the incision, and conversion of the light energy to heat by the LASE facilitated rapid photothermal sealing of the wound in approximately 1 min. Tissue repair with LASEs was evaluated using functional recovery (transepidermal water loss), biomechanical recovery (tensile strength), tissue visualization (ultrasound [US] and photoacoustic imaging [PAI]), and histology, and compared with that seen in sutures. Our studies indicate that LASEs promoted earlier recovery of barrier and mechanical function of healed skin compared to suture-closed incisions. Visualization of sealed skin using US and PAI indicated integration of the LASE with the tissue. Histological analyses of LASE-sealed skin sections showed reduced neutrophil and increased proresolution macrophages on Days 2 and 7 postclosure of incisions, without an increase in scarring or fibrosis. Together, our studies show that simple fabrication and application methods combined with rapid sealing of wound edges with improved histological outcomes make LASE a promising alternative for management of incisional wounds and lacerations.
Competing Interests: Kaushal Rege is affiliated with a start‐up company, Synergyan, LLC. Other authors declare no conflict of interest.
(© 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers.)
Databáze: MEDLINE
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