Reactive oxygen species turnover, phenolics metabolism, and some key gene expressions modulate postharvest physiological deterioration in cassava tubers.

Autor: Wahengbam ED; ICAR Research Complex for North Eastern Hill Region, Imphal, Manipur, India., Devi CP; ICAR Research Complex for North Eastern Hill Region, Imphal, Manipur, India., Sharma SK; ICAR Research Complex for North Eastern Hill Region, Imphal, Manipur, India., Roy SS; ICAR Research Complex for North Eastern Hill Region, Imphal, Manipur, India., Maibam A; ICAR Research Complex for North Eastern Hill Region, Imphal, Manipur, India., Dasgupta M; ICAR Research Complex for North Eastern Hill Region, Imphal, Manipur, India., Luikham S; ICAR Research Complex for North Eastern Hill Region, Imphal, Manipur, India., Chongtham T; ICAR Research Complex for North Eastern Hill Region, Imphal, Manipur, India., Ningombam A; ICAR Research Complex for North Eastern Hill Region, Imphal, Manipur, India., Bhupenchandra I; Farm Science Centre, ICAR Research Complex for North Eastern Hill Region, Imphal, Manipur, India., Singh LK; Farm Science Centre, ICAR Research Complex for North Eastern Hill Region, Imphal, Manipur, India., Devi YP; Farm Science Centre, ICAR Research Complex for North Eastern Hill Region, Imphal, Manipur, India., Thokchom S; Assam Agricultural University, Jorhat, Assam, India., Khaba CI; Assam Agricultural University, Jorhat, Assam, India., Singh NB; Institute of Bioresources and Sustainable Development, Imphal, Manipur, India., Rajashekar Y; Institute of Bioresources and Sustainable Development, Imphal, Manipur, India., Das S; Institute of Bioresources and Sustainable Development, Imphal, Manipur, India., Mohanty S; Central Horticultural Experiment Station, ICAR-Indian Institute of Horticultural Research, Bhubaneswar, Odisha, India., Sahoo MR; ICAR Research Complex for North Eastern Hill Region, Imphal, Manipur, India.; Central Horticultural Experiment Station, ICAR-Indian Institute of Horticultural Research, Bhubaneswar, Odisha, India.
Jazyk: angličtina
Zdroj: Frontiers in microbiology [Front Microbiol] 2023 Feb 28; Vol. 14, pp. 1148464. Date of Electronic Publication: 2023 Feb 28 (Print Publication: 2023).
DOI: 10.3389/fmicb.2023.1148464
Abstrakt: Rapid postharvest physiological deterioration (PPD) in cassava ( Manihot esculenta Crantz) tuber is a significant concern during storage. The freshly harvested tubers start spoiling within 24 to 72 h. Accumulation of H 2 O 2 is one of the earliest biochemical events that occurred during PPD, which was detected using the 3,3 diaminobenzidine (DAB) in two contrast cassava genotypes, MNP Local A (29-57 μg g -1 ) and Sree Prakash (64-141 μg g -1 ). Accumulating the fluorescence hydroxycoumarin compounds emitted by the cassava tubers observed under an ultraviolet (UV) lamp showed significant variations at 0, 3, 6, 9, 12, and 15 days of storage. The total phenolics and carotenoids significantly and negatively correlated with PPD progression; however, the anthocyanin and flavonoids positively correlated with the PPD-anchored ROS accumulation. The primary compound, Phthalic acid, di(2-propylpentyl) ester, was identified in both the cassava tubers, Sree Prakash (57.21 and 35.21%), and MNP Local A (75.58 and 60.21%) at 0, and 72 h of PPD, respectively. The expression of PPD-associated genes APX-2, APX-3, PAL , and AP was higher at 6-12 days of PPD, which signified the synthesis of ROS turnover and phenylpropanoid biosynthesis. A significant, strong, and positive correlation was established between the secondary metabolites and PPD signaling gene expression, which was inversely correlated with hydroxycoumarin and H 2 O 2 accumulation. MNP Local A tubers exhibited longer storage life of 15 days with a low PPD score, higher metabolites synthesis, and gene expression. The PPD-resistant lines may be used to augment cassava breeding strategies for large-scale commercial and industrial use.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Wahengbam, Devi, Sharma, Roy, Maibam, Dasgupta, Luikham, Chongtham, Ningombam, Bhupenchandra, Singh, Devi, Thokchom, Khaba, Singh, Rajashekar, Das, Mohanty and Sahoo.)
Databáze: MEDLINE
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