Antibody induction in mice by liposome-displayed recombinant enterotoxigenic Escherichia coli (ETEC) colonization antigens.

Autor:	Zhou S; Department of Biomedical Engineering, State University of New York at Buffalo, Buffalo, NY, USA., Yu KOA; Division of Pediatrics Infectious Diseases, Department of Pediatrics, University at Buffalo, Buffalo, NY, USA., Mabrouk MT; Department of Biomedical Engineering, State University of New York at Buffalo, Buffalo, NY, USA., Jahagirdar D; Department of Anatomy and Cell Biology, McGill University, Montreal, Canada., Huang WC; Department of Biomedical Engineering, State University of New York at Buffalo, Buffalo, NY, USA., Guerra JA; Division of Pediatrics Infectious Diseases, Department of Pediatrics, University at Buffalo, Buffalo, NY, USA., He X; Department of Biomedical Engineering, State University of New York at Buffalo, Buffalo, NY, USA., Ortega J; Department of Anatomy and Cell Biology, McGill University, Montreal, Canada., Poole ST; Naval Medical Research Center, Silver Spring, MD, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Hall ER; Naval Medical Research Center, Silver Spring, MD, USA., Gomez-Duarte OG; Division of Pediatrics Infectious Diseases, Department of Pediatrics, University at Buffalo, Buffalo, NY, USA., Maciel M Jr; Naval Medical Research Center, Silver Spring, MD, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA; Department of Microbiology and Immunology, Uniformed Services University Health System, Bethesda, MD, USA. Electronic address: milton.macieljr@gmail.com., Lovell JF; Department of Biomedical Engineering, State University of New York at Buffalo, Buffalo, NY, USA. Electronic address: jflovell@buffalo.edu.
Jazyk:	angličtina
Zdroj:	Biomedical journal [Biomed J] 2023 Dec; Vol. 46 (6), pp. 100588. Date of Electronic Publication: 2023 Mar 15.
DOI:	10.1016/j.bj.2023.03.001
Abstrakt:	Background: Enterotoxigenic Escherichia coli (ETEC) strains cause infectious diarrhea and colonize host intestine epithelia via surface-expressed colonization factors. Colonization factor antigen I (CFA/I), a prevalent ETEC colonization factor, is a vaccine target since antibodies directed to this fimbria can block ETEC adherence and prevent diarrhea. Methods: Two recombinant antigens derived from CFA/I were investigated with a vaccine adjuvant system that displays soluble antigens on the surface of immunogenic liposomes. The first antigen, CfaEB, is a chimeric fusion protein comprising the minor (CfaE) and major (CfaB) subunits of CFA/I. The second, CfaEad, is the adhesin domain of CfaE. Results: Owing to their His-tag, recombinant CfaEB and CfaEad, spontaneously bound upon admixture with nanoliposomes containing cobalt-porphyrin phospholipid (CoPoP), as well as a synthetic monophosphoryl lipid A (PHAD) adjuvant. Intramuscular immunization of mice with sub-microgram doses CfaEB or CfaEad admixed with CoPoP/PHAD liposomes elicited serum IgG and intestinal IgA antibodies. The smaller CfaEad antigen benefitted more from liposome display. Serum and intestine antibodies from mice immunized with liposome-displayed CfaEB or CfaEad recognized native CFA/I fimbria as evidenced by immunofluorescence and hemagglutination inhibition assays using the CFA/I-expressing H10407 ETEC strain. Conclusion: These data show that colonization factor-derived recombinant ETEC antigens exhibit immunogenicity when delivered in immunogenic particle-based formulations. Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Wei-Chiao Huang and Jonathan Lovell are named co-inventors on one or more University at Buffalo patent applications describing CoPoP technology and hold equity in POP Biotechnologies, a university startup company licensing the technology. (© 2023 Chang Gung University. Publishing services by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).)
Databáze:	MEDLINE
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