Comparison of the AmpFire and MA-6000 polymerase chain reaction platforms for high-risk human papillomavirus testing in cervical precancer screening.

Autor: Effah K; Catholic Hospital, Battor, P. O. Box 2, Battor, via Sogakope, Volta Region, Ghana., Agbemafle I; Fred N. Binka School of Public Health, University of Health and Allied Sciences, Ho, Ghana., Essel NO; Department of Emergency Medicine, College of Health Sciences, Faculty of Medicine and Dentistry, University of Alberta, 730 University Terrace, Edmonton AB T6G 2T4, Canada., Amuah JE; Catholic Hospital, Battor, P. O. Box 2, Battor, via Sogakope, Volta Region, Ghana; School of Epidemiology and Public Health, Faculty of Medicine, University of Ottawa, 451 Smyth Road (2046), Ottawa, ON K1H 8M5, Canada., Kotey EN; mPharma Ghana Ltd., No. 17 Garden Road, East Legon, Accra, Ghana., Antwi-Boasiako E; mPharma Ghana Ltd., No. 17 Garden Road, East Legon, Accra, Ghana., Kemawor S; Catholic Hospital, Battor, P. O. Box 2, Battor, via Sogakope, Volta Region, Ghana., Gedzah I; Catholic Hospital, Battor, P. O. Box 2, Battor, via Sogakope, Volta Region, Ghana., Tekpor E; Catholic Hospital, Battor, P. O. Box 2, Battor, via Sogakope, Volta Region, Ghana., Wormenor CM; Catholic Hospital, Battor, P. O. Box 2, Battor, via Sogakope, Volta Region, Ghana., Atuguba BH; Catholic Hospital, Battor, P. O. Box 2, Battor, via Sogakope, Volta Region, Ghana., Danyo S; Catholic Hospital, Battor, P. O. Box 2, Battor, via Sogakope, Volta Region, Ghana., Akakpo PK; Department of Pathology, University of Cape Coast, School of Medical Sciences, Clinical Teaching Center, Private Mail Bag, GN-06035819 Cape Coast, Ghana. Electronic address: k.p.akakpo@uccsms.edu.gh.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2023 Jun; Vol. 316, pp. 114709. Date of Electronic Publication: 2023 Mar 15.
DOI: 10.1016/j.jviromet.2023.114709
Abstrakt: High-risk human papillomavirus (hr-HPV) testing for primary cervical precancer screening offers an opportunity to improve screening in low-middle income countries (LMICs). This study aimed to compare the analytic performances of the AmpFire and MA-6000 platforms for hr-HPV DNA testing in three groups of women screened for hr-HPV types in Ghana: group 1 with 33 GeneXpert-archived ThinPrep/liquid-based samples subjected to both tests, group 2 with 50 AmpFire-archived dry brush samples subjected to MA-6000 testing, and group 3 involving 143 cotton swab samples simultaneously subjected to both tests without archiving. The overall agreement rates were 73 %, 92 %, and 84 %, for groups 1-3, respectively, and 84 % (95 % CI, 78.6-88.6) for the entire group. Neither AmpFire nor MA-6000 was more likely to test hr-HPV positive in all three groups and the combined group. Group 1 showed fair agreement without statistical significance (κ = 0.224, 95 % CI, -0.118 to 0.565), while group 3 showed significant moderate agreement (κ = 0.591, 95% CI, 0.442-0.741). Group 2 showed an almost perfect significant level of agreement (κ = 0.802; 95 % CI, 0.616-0.987). Thus, both platforms showed statistically significant moderate to near-perfect agreement for detecting hr-HPV in cervicovaginal samples, with variation according to archiving conditions and duration between sample collection and retesting. For LMICs using these platforms for COVID-19 testing, as the COVID-19 pandemic subsides, the platforms can become available for running other tests such as hr-HPV DNA testing for cervical precancer screening.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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