Color-resolved Cherenkov imaging allows for differential signal detection in blood and melanin content.

Autor:	Wickramasinghe VA; Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire, United States., Decker SM; Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire, United States., Streeter SS; Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire, United States., Sloop AM; Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire, United States., Petusseau AF; Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire, United States., Alexander DA; Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire, United States., Bruza P; Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire, United States., Gladstone DJ; Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire, United States.; Dartmouth College, Geisel School of Medicine, Department of Medicine, Hanover, New Hampshire, United States., Zhang R; Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire, United States.; Dartmouth College, Geisel School of Medicine, Department of Medicine, Hanover, New Hampshire, United States., Pogue BW; Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire, United States.; University of Wisconsin-Madison, Department of Medical Physics, Madison, Wisconsin, United States.
Jazyk:	angličtina
Zdroj:	Journal of biomedical optics [J Biomed Opt] 2023 Mar; Vol. 28 (3), pp. 036005. Date of Electronic Publication: 2023 Mar 13.
DOI:	10.1117/1.JBO.28.3.036005
Abstrakt:	Significance: High-energy x-ray delivery from a linear accelerator results in the production of spectrally continuous broadband Cherenkov light inside tissue. In the absence of attenuation, there is a linear relationship between Cherenkov emission and deposited dose; however, scattering and absorption result in the distortion of this linear relationship. As Cherenkov emission exits the absorption by tissue dominates the observed Cherenkov emission spectrum. Spectroscopic interpretation of this effects may help to better relate Cherenkov emission to ionizing radiation dose delivered during radiotherapy. Aim: In this study, we examined how color Cherenkov imaging intensity variations are caused by absorption from both melanin and hemoglobin level variations, so that future Cherenkov emission imaging might be corrected for linearity to delivered dose. Approach: A custom, time-gated, three-channel intensified camera was used to image the red, green, and blue wavelengths of Cherenkov emission from tissue phantoms with synthetic melanin layers and varying blood concentrations. Our hypothesis was that spectroscopic separation of Cherenkov emission would allow for the identification of attenuated signals that varied in response to changes in blood content versus melanin content, because of their different characteristic absorption spectra. Results: Cherenkov emission scaled with dose linearly in all channels. Absorption in the blue and green channels increased with increasing oxy-hemoglobin in the blood to a greater extent than in the red channel. Melanin was found to absorb with only slight differences between all channels. These spectral differences can be used to derive dose from measured Cherenkov emission. Conclusions: Color Cherenkov emission imaging may be used to improve the optical measurement and determination of dose delivered in tissues. Calibration for these factors to minimize the influence of the tissue types and skin tones may be possible using color camera system information based upon the linearity of the observed signals. (© 2023 The Authors.)
Databáze:	MEDLINE
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