Effects of long-term exposure to 50 Hz magnetic fields on cell viability, genetic damage, and sensitivity to mutagen-induced damage.

Autor:	Nguyen H; Scientific Direction Chemical and Physical Health Risks, Sciensano, 1050 Ixelles, Belgium.; Faculty of Medicine, Universite Libre de Bruxelles, 1070 Brussels, Belgium., Segers S; Scientific Direction Chemical and Physical Health Risks, Sciensano, 1050 Ixelles, Belgium., Ledent M; Scientific Direction Chemical and Physical Health Risks, Sciensano, 1050 Ixelles, Belgium., Anthonissen R; Scientific Direction Chemical and Physical Health Risks, Sciensano, 1050 Ixelles, Belgium., Verschaeve L; Scientific Direction Chemical and Physical Health Risks, Sciensano, 1050 Ixelles, Belgium., Hinsenkamp M; Faculty of Medicine, Universite Libre de Bruxelles, 1070 Brussels, Belgium., Collard JF; Faculty of Medicine, Universite Libre de Bruxelles, 1070 Brussels, Belgium., Feipel V; Faculty of Medicine, Universite Libre de Bruxelles, 1070 Brussels, Belgium., Mertens B; Scientific Direction Chemical and Physical Health Risks, Sciensano, 1050 Ixelles, Belgium.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2023 Feb 27; Vol. 9 (3), pp. e14097. Date of Electronic Publication: 2023 Feb 27 (Print Publication: 2023).
DOI:	10.1016/j.heliyon.2023.e14097
Abstrakt:	Until today, it remains controversial whether long-term exposure to extremely low-frequency magnetic fields (ELF-MF) below the legislative exposure limits could result in adverse human health effects. In the present study, the effects of long-term in vitro MF exposure on three different study endpoints (cell viability, genetic damage, and sensitivity to damage induced by known mutagens) were investigated in the human B lymphoblastoid (TK6) cell line. Cells were exposed to 50 Hz MF at three selected magnetic flux densities (i.e., 10, 100, and 500 μT) for different exposure periods ranging from 96h up to 6 weeks. Cell viability following MF exposure was assessed using the ATP-based cell viability assay. Effects of MF exposure on cell genetic damage and cell sensitivity to mutagen-induced damage were evaluated using the in vitro alkaline comet assay and the in vitro cytokinesis block micronucleus assay. The results showed that long-term exposure up to 96h to 50 Hz MF at all tested flux densities could significantly increase TK6 cell viability. In contrast, long-term MF exposure did not affect cell genetic damage, and long-term pre-exposure to MF did not change cell sensitivity to damage induced by known mutagens. At certain time points, statistically significant difference in genotoxicity test results were observed between the MF-exposed cells and the control cells. However, these observations could not be confirmed in the repeat experiments, indicating that they are probably not biologically significant. Competing Interests: The authors declare no competing interests. (© 2023 The Authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu