Synchrotron-Based X-ray Fluorescence Imaging Elucidates Uranium Toxicokinetics in Daphnia magna .

Autor: Byrnes I; Centre for Environmental Radioactivity (CERAD), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, P.O. Box 5003, 1433 Ås, Norway., Rossbach LM; Centre for Environmental Radioactivity (CERAD), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, P.O. Box 5003, 1433 Ås, Norway., Brede DA; Centre for Environmental Radioactivity (CERAD), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, P.O. Box 5003, 1433 Ås, Norway., Grolimund D; Swiss Light Source, Paul Scherrer Institute (PSI), 5232 Villigen, Switzerland., Ferreira Sanchez D; Swiss Light Source, Paul Scherrer Institute (PSI), 5232 Villigen, Switzerland., Nuyts G; AXIS Group, NANOlab Center of Excellence, Department of Physics, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium., Čuba V; Faculty of Nuclear Sciences and Physical Engineering, Czech Technical University in Prague, Brehova 7, 166 36 Prague 1, Czech Republic., Reinoso-Maset E; Centre for Environmental Radioactivity (CERAD), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, P.O. Box 5003, 1433 Ås, Norway., Salbu B; Centre for Environmental Radioactivity (CERAD), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, P.O. Box 5003, 1433 Ås, Norway., Janssens K; AXIS Group, NANOlab Center of Excellence, Department of Physics, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium., Oughton D; Centre for Environmental Radioactivity (CERAD), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, P.O. Box 5003, 1433 Ås, Norway., Scheibener S; Centre for Environmental Radioactivity (CERAD), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, P.O. Box 5003, 1433 Ås, Norway., Teien HC; Centre for Environmental Radioactivity (CERAD), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, P.O. Box 5003, 1433 Ås, Norway., Lind OC; Centre for Environmental Radioactivity (CERAD), Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, P.O. Box 5003, 1433 Ås, Norway.
Jazyk: angličtina
Zdroj: ACS nano [ACS Nano] 2023 Mar 28; Vol. 17 (6), pp. 5296-5305. Date of Electronic Publication: 2023 Mar 15.
DOI: 10.1021/acsnano.2c06111
Abstrakt: A combination of synchrotron-based elemental analysis and acute toxicity tests was used to investigate the biodistribution and adverse effects in Daphnia magna exposed to uranium nanoparticle (UNP, 3-5 nm) suspensions or to uranium reference (U ref ) solutions. Speciation analysis revealed similar size distributions between exposures, and toxicity tests showed comparable acute effects (UNP LC 50 : 402 μg L -1 [336-484], U ref LC 50 : 268 μg L -1 [229-315]). However, the uranium body burden was 3- to 5-fold greater in UNP-exposed daphnids, and analysis of survival as a function of body burden revealed a ∼5-fold higher specific toxicity from the U ref exposure. High-resolution X-ray fluorescence elemental maps of intact, whole daphnids from sublethal, acute exposures of both treatments revealed high uranium accumulation onto the gills (epipodites) as well as within the hepatic ceca and the intestinal lumen. Uranium uptake into the hemolymph circulatory system was inferred from signals observed in organs such as the heart and the maxillary gland. The substantial uptake in the maxillary gland and the associated nephridium suggests that these organs play a role in uranium removal from the hemolymph and subsequent excretion. Uranium was also observed associated with the embryos and the remnants of the chorion, suggesting uptake in the offspring. The identification of target organs and tissues is of major importance to the understanding of uranium and UNP toxicity and exposure characterization that should ultimately contribute to reducing uncertainties in related environmental impact and risk assessments.
Databáze: MEDLINE
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