Calcium imaging and analysis of the jugular-nodose ganglia enables identification of distinct vagal sensory neuron subsets.

Autor:	Huerta TS; Laboratory for Biomedical Sciences, Institute for Bioelectronic Medicine, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, United States of America.; Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, United States of America., Haider B; Laboratory for Biomedical Sciences, Institute for Bioelectronic Medicine, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, United States of America., Adamovich-Zeitlin R; Laboratory for Biomedical Sciences, Institute for Bioelectronic Medicine, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, United States of America.; Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, United States of America., Chen AC; Laboratory for Biomedical Sciences, Institute for Bioelectronic Medicine, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, United States of America.; Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, United States of America., Chaudhry S; Laboratory for Biomedical Sciences, Institute for Bioelectronic Medicine, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, United States of America., Zanos TP; Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, United States of America.; Institute of Health System Science, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, United States of America.; Elmezzi Graduate School of Molecular Medicine, Manhasset, NY, United States of America., Chavan SS; Laboratory for Biomedical Sciences, Institute for Bioelectronic Medicine, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, United States of America.; Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, United States of America.; Elmezzi Graduate School of Molecular Medicine, Manhasset, NY, United States of America., Tracey KJ; Laboratory for Biomedical Sciences, Institute for Bioelectronic Medicine, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, United States of America.; Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, United States of America.; Elmezzi Graduate School of Molecular Medicine, Manhasset, NY, United States of America., Chang EH; Laboratory for Biomedical Sciences, Institute for Bioelectronic Medicine, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, United States of America.; Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, United States of America.; Elmezzi Graduate School of Molecular Medicine, Manhasset, NY, United States of America.
Jazyk:	angličtina
Zdroj:	Journal of neural engineering [J Neural Eng] 2023 Mar 15; Vol. 20 (2). Date of Electronic Publication: 2023 Mar 15.
DOI:	10.1088/1741-2552/acbe1e
Abstrakt:	Objective. Sensory nerves of the peripheral nervous system (PNS) transmit afferent signals from the body to the brain. These peripheral nerves are composed of distinct subsets of fibers and associated cell bodies, which reside in peripheral ganglia distributed throughout the viscera and along the spinal cord. The vagus nerve (cranial nerve X) is a complex polymodal nerve that transmits a wide array of sensory information, including signals related to mechanical, chemical, and noxious stimuli. To understand how stimuli applied to the vagus nerve are encoded by vagal sensory neurons in the jugular-nodose ganglia, we developed a framework for micro-endoscopic calcium imaging and analysis. Approach. We developed novel methods for in vivo imaging of the intact jugular-nodose ganglion using a miniature microscope (Miniscope) in transgenic mice with the genetically-encoded calcium indicator GCaMP6f. We adapted the Python-based analysis package Calcium Imaging Analysis (CaImAn) to process the resulting one-photon fluorescence data into calcium transients for subsequent analysis. Random forest classification was then used to identify specific types of neuronal responders. Results. We demonstrate that recordings from the jugular-nodose ganglia can be accomplished through careful surgical dissection and ganglia stabilization. Using a customized acquisition and analysis pipeline, we show that subsets of vagal sensory neurons respond to different chemical stimuli applied to the vagus nerve. Successful classification of the responses with a random forest model indicates that certain calcium transient features, such as amplitude and duration, are important for encoding these stimuli by sensory neurons. Significance. This experimental approach presents a new framework for investigating how individual vagal sensory neurons encode various stimuli on the vagus nerve. Our surgical and analytical approach can be applied to other PNS ganglia in rodents and other small animal species to elucidate previously unexplored roles for peripheral neurons in a diverse set of physiological functions. (Creative Commons Attribution license.)
Databáze:	MEDLINE
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