Circ_0007429/miR-637/TRIM71/Ago2 axis participates in the regulation of proliferation, migration, invasion, apoptosis, and aerobic glycolysis of HCC.

Autor: Fan L; Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, China.; Hubei Clinical Center & Key Laboratory of Intestinal & Colorectal Diseases, Wuhan, China., Xia P; Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, China.; Hubei Clinical Center & Key Laboratory of Intestinal & Colorectal Diseases, Wuhan, China., Wang J; Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, China.; Hubei Clinical Center & Key Laboratory of Intestinal & Colorectal Diseases, Wuhan, China., Xu S; Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, China.; Hubei Clinical Center & Key Laboratory of Intestinal & Colorectal Diseases, Wuhan, China., Qiu Z; Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, China.; Hubei Clinical Center & Key Laboratory of Intestinal & Colorectal Diseases, Wuhan, China., Wu Y; Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, China.; Hubei Clinical Center & Key Laboratory of Intestinal & Colorectal Diseases, Wuhan, China., Feng M; Department of Gastrointerstinal Surgery, Zhongnan Hospital of Wuhan University, Wuhan, China.; Clinical Cancer Study Center of Hubei Province, Wuhan, China.; Key Laboratory of Tumor Biological Behavior of Hubei Province, Wuhan, China., Zhao Q; Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, China.; Hubei Clinical Center & Key Laboratory of Intestinal & Colorectal Diseases, Wuhan, China., Wang H; Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, China.; Hubei Clinical Center & Key Laboratory of Intestinal & Colorectal Diseases, Wuhan, China., Li X; Department of Gastrointerstinal Surgery, Zhongnan Hospital of Wuhan University, Wuhan, China.; Clinical Cancer Study Center of Hubei Province, Wuhan, China.; Key Laboratory of Tumor Biological Behavior of Hubei Province, Wuhan, China.
Jazyk: angličtina
Zdroj: Molecular carcinogenesis [Mol Carcinog] 2023 Jun; Vol. 62 (6), pp. 820-832. Date of Electronic Publication: 2023 Mar 15.
DOI: 10.1002/mc.23526
Abstrakt: CircRNAs play an important role in the progression of hepatocellular carcinoma (HCC), however, the role of circ_0007429 in HCC remains unknown. Using bioinformatics tools, we selected circ_0007429 that was most highly expressed in HCC tissues and investigated its role in HCC progression. Immunohistochemistry, plasmid transfection, real-time quantitative PCR, and western blot analysis were used to identify the relationship between circ_0007429 and its potential target, miR-637, and TRIM71. The regulatory effect of circ_0007429 on miR-637/TRIM71/Ago2 signaling and its key role in HCC progression were studied in vitro. A nude mouse xenograft model was used to examine tumor growth in vivo. Circ_0007429 and TRIM71 expression were upregulated, while miR-637 expression was downregulated in HCC tissues and cells compared with their expression in control groups. Knockdown of circ_0007429 enhanced apoptosis in HCC cells, while impeded proliferation, migration, invasion, and aerobic glycolysis, which were reversed by miR-637 inhibitor. High levels of circ_0007429 correlated with a poor survival rate of HCC patients. Additionally, circ_0007429 interfering inhibited tumor growth in vivo. TRIM71 directly bound to miR-637 and inhibited Ago2 expression. Moreover, circ_0007429 promotes aerobic glycolysis in HCC cells through the miR/TRIM71/Ago2 axis. Circ_0007429 promotes HCC progression by promoting cell proliferation, migration, invasion, and aerobic glycolysis and by inhibiting cell apoptosis through the miR/TRIM71/Ago2 axis. These results provide molecular insights into the mechanism of HCC and suggest that circ_0007429 could be a therapeutic target for HCC.
(© 2023 The Authors. Molecular Carcinogenesis published by Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: