Clindamycin phosphate and bone morphogenetic protein-7 loaded combined nanoparticle-graft and nanoparticle-film formulations for alveolar bone regeneration - An in vitro and in vivo evaluation.

Autor: Ilhan M; Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Technology, 06560 Ankara, Türkiye; Duzce University, Faculty of Pharmacy, Department of Pharmaceutical Technology, 81620 Duzce, Türkiye. Electronic address: mirayilhan@duzce.edu.tr., Kilicarslan M; Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Technology, 06560 Ankara, Türkiye. Electronic address: cmkilicarslan@gmail.com., Alcigir ME; Kirikkale University, Faculty of Veterinary Medicine, Department of Pathology, 71450 Kirikkale, Türkiye. Electronic address: erayalcigir@kku.edu.tr., Bagis N; Ankara University, Faculty of Dentistry, Department of Periodontology, 06560 Ankara, Türkiye. Electronic address: nbagis@ankara.edu.tr., Ekim O; Ankara University, Faculty of Veterinary Medicine, Department of Anatomy, 06110 Ankara, Türkiye. Electronic address: ekim@ankara.edu.tr., Orhan K; Ankara University, Faculty of Dentistry, Department of Dentomaxillofacial Radiology, 06560 Ankara, Türkiye. Electronic address: Kaan.Orhan@dentistry.ankara.edu.tr.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2023 Apr 05; Vol. 636, pp. 122826. Date of Electronic Publication: 2023 Mar 12.
DOI: 10.1016/j.ijpharm.2023.122826
Abstrakt: Commonly utilized techniques for healing alveolar bone destruction such as the use of growth factors, suffering from short half-life, application difficulties, and the ability to achieve bioactivity only in the presence of high doses of growth factor. The sustained release of growth factors through a scaffold-based delivery system offers a promising and innovative tool in dentistry. Furthermore, it is suggested to guide the host response by using antimicrobials together with growth factors to prevent recovery and achieve ideal regeneration. Herein, the aim was to prepare and an in vitro - in vivo evaluation of bone morphogenetic protein 7 (BMP-7) and clindamycin phosphate (CDP) loaded polymeric nanoparticles, and their loading into the alginate-chitosan polyelectrolyte complex film or alloplastic graft to accelerate hard tissue regeneration. PLGA nanoparticles containing CDP and BMP-7, separately or together, were prepared using the double emulsion solvent evaporation technique. Through in vitro assays, it was revealed that spherical particles were homogeneously distributed in the combination formulations, and sustained release could be achieved for >12 weeks with all formulations. Also, results from the micro-CT and histopathological analyses indicated that CDP and BMP-7 loaded nanoparticle-film formulations were more effective in treatment than the nanoparticle loaded grafts.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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