An Optrode Array for Spatiotemporally Precise Large-Scale Optogenetic Stimulation of Deep Cortical Layers in Non-human Primates.

Autor:	Angelucci A; Moran Eye Institute, University of Utah Health., Clark A; Moran Eye Institute, University of Utah Health., Ingold A; Moran Eye Institute, University of Utah Health., Reiche C; University of Utah., Cundy D 3rd; Moran Eye Institute, University of Utah Health., Balsor J; Moran Eye Institute, University of Utah Health., Federer F; Moran Eye Institute, University of Utah Health., McAlinden N; University of Strathclyde., Cheng Y; University of Strathclyde., Rolston J; Brigham & Women's Hospital and Harvard Medical School., Rieth L; West Virginia University., Dawson M; University of Strathclyde., Mathieson K; University of Strathclyde., Blair S; University of Utah.
Jazyk:	angličtina
Zdroj:	Research square [Res Sq] 2023 Feb 28. Date of Electronic Publication: 2023 Feb 28.
DOI:	10.21203/rs.3.rs-2322768/v1
Abstrakt:	Optogenetics has transformed studies of neural circuit function, but remains challenging to apply in non-human primates (NHPs). A major challenge is delivering intense and spatially precise patterned photostimulation across large volumes in deep tissue. Here, we have developed and validated the Utah Optrode Array (UOA) to meet this critical need. The UOA is a 10×10 glass waveguide array bonded to an electrically-addressable μLED array. In vivo electrophysiology and immediate early gene (c-fos) immunohistochemistry demonstrated the UOA allows for large-scale spatiotemporally precise neuromodulation of deep tissue in macaque primary visual cortex. Specifically, the UOA permits both focal (single layers or columns), and large-scale (across multiple layers or columns) photostimulation of deep cortical layers, simply by varying the number of simultaneously activated μLEDs and/or the light irradiance. These results establish the UOA as a powerful tool for studying targeted neural populations within single or across multiple deep layers in complex NHP circuits. Competing Interests: COMPETING INTERESTS STATEMENT The authors declare no competing interests.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu