Genomic and metabolic properties of Staphylococcus gallinarum FCW1 MCC4687 isolated from naturally fermented coconut water towards GRAS assessment.

Autor: Dhanya Raj CT; Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India., Kandaswamy S; School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, Lancashire PR1 2HE, United Kingdom; Manchester Centre for Genomic Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, 6(th) Floor, St Mary's Hospital, Oxford Road, Manchester M13 9WL, United Kingdom., Suryavanshi MV; Cardiovascular and Metabolic Sciences Department, Lerner Research Institute, Cleveland Clinic, OH 44195, United States. Electronic address: suryavm@ccf.org., Ramasamy KP; Department of Ecology and Environmental Science, Umeå University, 901 87 Umeå, Sweden., Rajasabapathy R; Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India., Arthur James R; Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India. Electronic address: james@bdu.ac.in.
Jazyk: angličtina
Zdroj: Gene [Gene] 2023 May 30; Vol. 867, pp. 147356. Date of Electronic Publication: 2023 Mar 11.
DOI: 10.1016/j.gene.2023.147356
Abstrakt: Staphylococcus gallinarum FCW1 was isolated from naturally fermented coconut-water and identified by biochemical and molecular methods. Probiotic characterization and safety assessment were conducted through a series of in vitro tests. A high survival rate was observed when the strain was tested for resistance to bile, lysozyme, simulated gastric and intestinal fluid, phenol, and different temperature and salt concentrations. The strain showed antagonism against some pathogens, was susceptible to all antibiotics tested except penicillin, and showed no hemolytic and DNase activity. Hydrophobicity, autoaggregation, biofilm formation, and antioxidation tests indicated that the strain possessed a high adhesive and antioxidant ability. Enzymatic activity was used to evaluate the metabolic capacities of the strain. In-vivo experiment on zebrafish was performed to check its safety status. The whole-genome sequencing indicated that the genome contained 2,880,305 bp with a GC content of 33.23%. The genome annotation confirmed the presence of probiotic-associated genes and genes for oxalate degradation, sulfate reduction, acetate metabolism, and ammonium transport in the FCW1 strain, adding to the theory that this strain may be helpful in treating kidney stones. This study revealed that the strain FCW1 might be an excellent potential probiotic in developing fermented coconut beverages and treating and preventing kidney stone disease.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE