Photodynamic antimicrobial therapy (A-PDT) using 1,9-Dimethyl-Methylene Blue zinc chloride double salt - DMMB and λ640 ± 5ηm LED light in patients undertaking orthodontic treatment.

Autor: Soares LGP; Center of Biophotonics, School of Dentistry, Federal University of Bahia, 62, Araújo Pinho Ave, Canela, Salvador, BA CEP 40110-150, Brazil; Center for Orthodontics and Facial Orthopedics Prof. José Édimo Soares Martins, School of Dentistry, Federal University of Bahia, Salvador, BA CEP 40110-150, Brazil., de Almeida CG; Center for Orthodontics and Facial Orthopedics Prof. José Édimo Soares Martins, School of Dentistry, Federal University of Bahia, Salvador, BA CEP 40110-150, Brazil., Crugeira PJL; Center of Biophotonics, School of Dentistry, Federal University of Bahia, 62, Araújo Pinho Ave, Canela, Salvador, BA CEP 40110-150, Brazil; Instituto Politécnico de Bragança, Campus de Santa Apolónia, Bragança 5300-253, Portugal; Laboratory of Biotechnology and Ecology of Micro-organisms, Institute of Health Science, Federal University of Bahia, Salvador, BA CEP 40110-100, Brazil., Nunes IPF; Center of Biophotonics, School of Dentistry, Federal University of Bahia, 62, Araújo Pinho Ave, Canela, Salvador, BA CEP 40110-150, Brazil., da Silva APLT; Center of Biophotonics, School of Dentistry, Federal University of Bahia, 62, Araújo Pinho Ave, Canela, Salvador, BA CEP 40110-150, Brazil., Almeida JA; Center of Biophotonics, School of Dentistry, Federal University of Bahia, 62, Araújo Pinho Ave, Canela, Salvador, BA CEP 40110-150, Brazil. Electronic address: jeovana.almeida@ufba.br., Cangussú MCT; Center of Biophotonics, School of Dentistry, Federal University of Bahia, 62, Araújo Pinho Ave, Canela, Salvador, BA CEP 40110-150, Brazil; Epidemiology and Public Health, School of Dentistry, Federal University of Bahia, Salvador, BA CEP 40110-150, Brazil. Electronic address: cangussu@ufba.br., de Almeida PF; Department of Biointeraction, Institute of Health Science, Federal University of Bahia, Salvador, BA CEP 40110-100, Brazil; Laboratory of Biotechnology and Ecology of Micro-organisms, Institute of Health Science, Federal University of Bahia, Salvador, BA CEP 40110-100, Brazil. Electronic address: pfa@ufba.br., Habib FAL; Center for Orthodontics and Facial Orthopedics Prof. José Édimo Soares Martins, School of Dentistry, Federal University of Bahia, Salvador, BA CEP 40110-150, Brazil. Electronic address: fhabib@terra.com.br., Pinheiro ALB; Center of Biophotonics, School of Dentistry, Federal University of Bahia, 62, Araújo Pinho Ave, Canela, Salvador, BA CEP 40110-150, Brazil. Electronic address: albp@ufba.br.
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2023 Jun; Vol. 42, pp. 103503. Date of Electronic Publication: 2023 Mar 11.
DOI: 10.1016/j.pdpdt.2023.103503
Abstrakt: Orthodontic treatment involves the use of apparatuses that impairs oral hygiene making patients susceptible to periodontal diseases and caries. To prevent increased antimicrobial resistance A-PDT has shown itself a feasible option. The aim of this investigation was to assess the efficiency of A-PDT employing 1,9-Dimethyl-Methylene Blue zinc chloride double salt - DMMB as a photosensitizing agent combined with red LED irradiation (λ640 ± 5 ηm) against oral biofilm of patients undertaking orthodontic treatment. Twenty-one patients agreed to participate. Four biofilm collections were carried out on brackets and gingiva around inferior central incisors; first was carried out before any treatment (Control); second followed five minutes of pre-irradiation, the third was immediately after the first AmPDT, and the last after a second AmPDT. Then, a microbiological routine for microorganism growth was carried out and, after 24-h, CFU counting was performed. There was significant difference between all groups. No significant difference was seen between Control and Photosensitizer and AmpDT1 and AmPDT2 groups. Significant differences were observed between Control and AmPDT1 and AmPDT2 groups, Photosensitizer and AmPDT1 and AmPDT2 groups. It was concluded that double AmPDT using DMBB in nano concentration and red LED was capable to meaningfully decrease the number of CFUs in orthodontic patients.
Competing Interests: Declaration of Competing Interest None
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: