Characterization of Dendritic Cell Metabolism by Flow Cytometry.
| Autor: | Brombacher EC; Department of Parasitology, Leiden University Medical Centre, Leiden, Netherlands., Patente TA; Department of Parasitology, Leiden University Medical Centre, Leiden, Netherlands., Quik M; Department of Parasitology, Leiden University Medical Centre, Leiden, Netherlands., Everts B; Department of Parasitology, Leiden University Medical Centre, Leiden, Netherlands. b.everts@lumc.nl. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2618, pp. 219-237. |
| DOI: | 10.1007/978-1-0716-2938-3_16 |
| Abstrakt: | In response to different stimuli, dendritic cells (DCs) undergo metabolic reprogramming to support their function. Here we describe how fluorescent dyes and antibody-based approaches can be used to assess various metabolic parameters of DCs including glycolysis, lipid metabolism, mitochondrial activity, and the activity of important sensors and regulators of cellular metabolism, mTOR and AMPK. These assays can be performed using standard flow cytometry and will allow for the determination of metabolic properties of DC populations at single-cell level and to characterize metabolic heterogeneity within them. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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