| Autor: |
Banerjee S; Ames Laboratory, U.S. Department of Energy, Ames, IA 50011, USA., Hemmat MA; Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA., Shubham S; Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA., Gosai A; Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA., Devarakonda S; Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA., Jiang N; Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA., Geekiyanage C; Aptalogic Inc., Ames, IA 50014, USA., Dillard JA; Department of Microbiology and Immunology, University of Iowa, Iowa City, IA 50011, USA., Maury W; Department of Microbiology and Immunology, University of Iowa, Iowa City, IA 50011, USA., Shrotriya P; Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, USA., Lamm MH; Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA., Nilsen-Hamilton M; Ames Laboratory, U.S. Department of Energy, Ames, IA 50011, USA.; Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA.; Aptalogic Inc., Ames, IA 50014, USA. |
| Abstrakt: |
The Ebola virus glycoprotein (GP) gene templates several mRNAs that produce either the virion-associated transmembrane protein or one of two secreted glycoproteins. Soluble glycoprotein (sGP) is the predominant product. GP1 and sGP share an amino terminal sequence of 295 amino acids but differ in quaternary structure, with GP1 being a heterohexamer with GP2 and sGP a homodimer. Two structurally different DNA aptamers were selected against sGP that also bound GP1,2. These DNA aptamers were compared with a 2'FY-RNA aptamer for their interactions with the Ebola GP gene products. The three aptamers have almost identical binding isotherms for sGP and GP1,2 in solution and on the virion. They demonstrated high affinity and selectivity for sGP and GP1,2. Furthermore, one aptamer, used as a sensing element in an electrochemical format, detected GP1,2 on pseudotyped virions and sGP with high sensitivity in the presence of serum, including from an Ebola-virus-infected monkey. Our results suggest that the aptamers interact with sGP across the interface between the monomers, which is different from the sites on the protein bound by most antibodies. The remarkable similarity in functional features of three structurally distinct aptamers suggests that aptamers, like antibodies, have preferred binding sites on proteins. |
