| Autor: |
Agarwal A; Environmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers University, New Brunswick, NJ 08901.; Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, NJ 08901., Levitan O; Environmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers University, New Brunswick, NJ 08901.; Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, NJ 08901., Cruz de Carvalho H; Institut de Biologie de l'ENS, Ecole normale supérieure, CNRS, Inserm, Université Paris Sciences & Letters, Paris 75005, France.; Faculté des Sciences et Technologie, Université Paris Est-Créteil 94000 Créteil, France., Falkowski PG; Environmental Biophysics and Molecular Ecology Program, Department of Marine and Coastal Sciences, Rutgers University, New Brunswick, NJ 08901.; Department of Earth and Planetary Sciences, Rutgers University, Piscataway, NJ 08854. |
| Abstrakt: |
Unlike most higher plants, unicellular algae can acclimate to changes in irradiance on time scales of hours to a few days. The process involves an enigmatic signaling pathway originating in the plastid that leads to coordinated changes in plastid and nuclear gene expression. To deepen our understanding of this process, we conducted functional studies to examine how the model diatom, Phaeodactylum tricornutum, acclimates to low light and sought to identify the molecules responsible for the phenomenon. We show that two transformants with altered expression of two putative signal transduction molecules, a light-specific soluble kinase and a plastid transmembrane protein, that appears to be regulated by a long noncoding natural antisense transcript, arising from the opposite strand, are physiologically incapable of photoacclimation. Based on these results, we propose a working model of the retrograde feedback in the signaling and regulation of photoacclimation in a marine diatom. |
