Artificial feeding of Ornithodoros rostratus using a silicone membrane system.

Autor: Ribeiro CCDU; Veterinary Sciences Post Graduation Program, Federal Rural University of Rio de Janeiro - UFRRJ, Seropédica, RJ, Brasil., Cordeiro MD; Practice in Sustainable Development Post Graduation Program, Federal Rural University of Rio de Janeiro - UFRRJ, Seropédica, RJ, Brasil. mathcordeiro@hotmail.com.; Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Br 465, Km 7, Seropédica, Rio de Janeiro, 23891-000, Brazil. mathcordeiro@hotmail.com., Cepeda PB; Veterinary Sciences Post Graduation Program, Federal Rural University of Rio de Janeiro - UFRRJ, Seropédica, RJ, Brasil., Valim JRA; Veterinary Sciences Post Graduation Program, Federal Rural University of Rio de Janeiro - UFRRJ, Seropédica, RJ, Brasil., Fonseca AH; Veterinary Sciences Post Graduation Program, Federal Rural University of Rio de Janeiro - UFRRJ, Seropédica, RJ, Brasil., de Azevedo Baêta B; Veterinary Sciences Post Graduation Program, Federal Rural University of Rio de Janeiro - UFRRJ, Seropédica, RJ, Brasil.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2023 May; Vol. 122 (5), pp. 1213-1219. Date of Electronic Publication: 2023 Mar 10.
DOI: 10.1007/s00436-023-07821-7
Abstrakt: The in vitro feeding of ticks facilitates the conduction of studies involving the intrinsic vector-pathogen relationship, susceptibility tests, and resistance to acaricides, in addition to mimicking the use of experimental hosts. The objective of this study was to establish an in vitro feeding system using silicone membranes to supply various diets to the species Ornithodoros rostratus. Each experimental group included 130 first-instar O. rostratus nymphs. The groups were divided according to the diet provided: citrated rabbit blood, citrated bovine blood, bovine blood with antibiotics, and defibrinated bovine blood. The control group was fed directly on rabbits. Ticks were weighed before and after the feeding and monitored individually according to their biological parameters. The results of the experiment demonstrated that the proposed system was efficient in terms of fixation stimulus and satisfactory in terms of tick engorgement, which would allow the maintenance of O. rostratus colonies by using artificial feeding through silicone membranes. All diets provided were efficient for the maintenance of colonies, but the ticks that received citrated rabbit blood displayed similar biological parameters to those observed under in vivo feeding conditions.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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