| Autor: |
Dósa Z; Department of Biomedicine, Aarhus University, Aarhus, Denmark., Nieto-Gonzalez JL; Department of Biomedicine, Aarhus University, Aarhus, Denmark., Elfving B; Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Aarhus, Denmark., Hougaard KS; National Research Centre for the Working Environment, Copenhagen, Denmark.; Department of Public Health, University of Copenhagen, Copenhagen, Denmark., Holm MM; Department of Biomedicine, Aarhus University, Aarhus, Denmark., Wegener G; Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.; Pharmaceutical Research Center of Excellence, North-West University, Potchefstroom, South Africa., Jensen K; Department of Biomedicine, Aarhus University, Aarhus, Denmark.; Department of Neurology, Aalborg University Hospital, Aalborg, Denmark. |
| Abstrakt: |
Prenatal stress is believed to increase the risk of developing neuropsychiatric disorders, including major depression. Adverse genetic and environmental impacts during early development, such as glucocorticoid hyper-exposure, can lead to changes in the foetal brain, linked to mental illnesses developed in later life. Dysfunction in the GABAergic inhibitory system is associated with depressive disorders. However, the pathophysiology of GABAergic signalling is poorly understood in mood disorders. Here, we investigated GABAergic neurotransmission in the low birth weight (LBW) rat model of depression. Pregnant rats, exposed to dexamethasone, a synthetic glucocorticoid, during the last week of gestation, yielded LBW offspring showing anxiety- and depressive-like behaviour in adulthood. Patch-clamp recordings from dentate gyrus granule cells in brain slices were used to examine phasic and tonic GABA A receptor-mediated currents. The transcriptional levels of selected genes associated with synaptic vesicle proteins and GABAergic neurotransmission were investigated. The frequency of spontaneous inhibitory postsynaptic currents (sIPSC) was similar in control and LBW rats. Using a paired-pulse protocol to stimulate GABAergic fibres impinging onto granule cells, we found indications of decreased probability of GABA release in LBW rats. However, tonic GABAergic currents and miniature IPSCs, reflecting quantal vesicle release, appeared normal. Additionally, we found elevated expression levels of two presynaptic proteins, Snap-25 and Scamp2 , components of the vesicle release machinery. The results suggest that altered GABA release may be an essential feature in the depressive-like phenotype of LBW rats. |
