Time-Dependent Changes in the Biofluid Levels of Neural Injury Markers in Severe Traumatic Brain Injury Patients-Cerebrospinal Fluid and Cerebral Microdialysates: A Longitudinal Prospective Pilot Study.

Autor:	Lin IH; Department of Anatomy, Physiology and Genetics, Uniformed Services University, Bethesda, Maryland, USA., Kamnaksh A; Department of Anatomy, Physiology and Genetics, Uniformed Services University, Bethesda, Maryland, USA., Aniceto R; Department of Anatomy, Physiology and Genetics, Uniformed Services University, Bethesda, Maryland, USA., McCullough J; Department of Anatomy, Physiology and Genetics, Uniformed Services University, Bethesda, Maryland, USA., Bekdash R; Department of Anatomy, Physiology and Genetics, Uniformed Services University, Bethesda, Maryland, USA., Eklund M; Department of Anatomy, Physiology and Genetics, Uniformed Services University, Bethesda, Maryland, USA., Ghatan PH; Department of Neuroscience, Uppsala University Hospital, Uppsala, Sweden., Risling M; Department of Neuroscience, Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden., Svensson M; Department of Clinical Neuroscience, Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.; Department of Neurosurgery, Karolinska University Hospital, Stockholm, Sweden., Bellander BM; Department of Clinical Neuroscience, Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.; Department of Neurosurgery, Karolinska University Hospital, Stockholm, Sweden., Nelson DW; Department of Perioperative Medicine and Intensive Care, Karolinska University Hospital, Stockholm, Sweden.; Section of Perioperative Medicine and Intensive Care, Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden., Thelin EP; Department of Clinical Neuroscience, Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.; Department of Neurology, Karolinska University Hospital, Stockholm, Sweden., Agoston DV; Department of Anatomy, Physiology and Genetics, Uniformed Services University, Bethesda, Maryland, USA.
Jazyk:	angličtina
Zdroj:	Neurotrauma reports [Neurotrauma Rep] 2023 Mar 01; Vol. 4 (1), pp. 107-117. Date of Electronic Publication: 2023 Mar 01 (Print Publication: 2023).
DOI:	10.1089/neur.2022.0076
Abstrakt:	Monitoring protein biomarker levels in the cerebrospinal fluid (CSF) can help assess injury severity and outcome after traumatic brain injury (TBI). Determining injury-induced changes in the proteome of brain extracellular fluid (bECF) can more closely reflect changes in the brain parenchyma, but bECF is not routinely available. The aim of this pilot study was to compare time-dependent changes of S100 calcium-binding protein B (S100B), neuron-specific enolase (NSE), total Tau, and phosphorylated Tau (p-Tau) levels in matching CSF and bECF samples collected at 1, 3, and 5 days post-injury from severe TBI patients ( n  = 7; GCS 3-8) using microcapillary-based western analysis. We found that time-dependent changes in CSF and bECF levels were most pronounced for S100B and NSE, but there was substantial patient-to-patient variability. Importantly, the temporal pattern of biomarker changes in CSF and bECF samples showed similar trends. We also detected two different immunoreactive forms of S100B in both CSF and bECF samples, but the contribution of the different immunoreactive forms to total immunoreactivity varied from patient to patient and time point to time point. Our study is limited, but it illustrates the value of both quantitative and qualitative analysis of protein biomarkers and the importance of serial sampling for biofluid analysis after severe TBI. Competing Interests: No competing financial interests exist. (© I-Hsuan Lin et al., 2023; Published by Mary Ann Liebert, Inc.)
Databáze:	MEDLINE
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