Isolation of a Population of Cells Co-Expressing Markers of Embryonic Stem Cells and Mesenchymal Stem Cells from the Rudimentary Uterine Horn of a Patient with Uterine Aplasia.

Autor: Burunova VV; V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia. vburunova@mail.ru.; LLC ' NPC Stemma', Moscow, Russia. vburunova@mail.ru., Gisina AM; V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia., Yarygina NK; V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia., Sukhinich KK; V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.; N. K. Kol'-tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia., Makiyan ZN; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia., Yarygin KN; V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2023 Feb; Vol. 174 (4), pp. 549-555. Date of Electronic Publication: 2023 Mar 10.
DOI: 10.1007/s10517-023-05746-w
Abstrakt: More than 50% cells isolated from the endometrial cavity scraping and the myometrium of the rudimentary horn of an underdeveloped uterus removed from a patient with uterine aplasia and maintained under culturing conditions normal for mesenchymal stem cells (MSC) expressed embryonic transcription factors Oct4 and Nanog, embryonic cell membrane sialyl glycolipid SSEA4, and MSC markers. After 2-3 passages, the cells lost the expression of the early embryogenesis markers, but retained MSC markers. The presence of dormant stem cells in the underdeveloped endometrium and in the uterus indicates that this tissue has a regenerative potential that can be activated and used for completion of organ morphogenesis. This task requires the development of methods of early diagnosis of morphogenesis impairment and tools for safe reactivation of the ontogenesis.
(© 2023. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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