Cre-dependent ACR2-expressing reporter mouse strain for efficient long-lasting inhibition of neuronal activity.

Autor: Mukai Y; Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya, 464-8601, Japan.; Department of Neural Regulation, Nagoya University Graduate School of Medicine, Nagoya, 466-8550, Japan., Li Y; Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya, 464-8601, Japan.; Department of Neural Regulation, Nagoya University Graduate School of Medicine, Nagoya, 466-8550, Japan., Nakamura A; Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya, 464-8601, Japan., Fukatsu N; Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya, 464-8601, Japan., Iijima D; Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya, 464-8601, Japan., Abe M; Department of Animal Model Development, Brain Research Institute, Niigata University, Niigata, 951-8585, Japan., Sakimura K; Department of Animal Model Development, Brain Research Institute, Niigata University, Niigata, 951-8585, Japan., Itoi K; Department of Nursing, Faculty of Health Sciences, Tohoku Fukushi University, Sendai, 981-8522, Japan., Yamanaka A; Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya, 464-8601, Japan. yamank@cibr.ac.cn.; Department of Neural Regulation, Nagoya University Graduate School of Medicine, Nagoya, 466-8550, Japan. yamank@cibr.ac.cn.; Chinese Institute for Brain Research, Beijing, 102206, China. yamank@cibr.ac.cn.; National Institute for Physiological Sciences, National Institutes of Natural Sciences, Aichi, 444-8585, Japan. yamank@cibr.ac.cn.; Department of Neuropsychiatry, Keio University School of Medicine, Tokyo, 160-8582, Japan. yamank@cibr.ac.cn.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Mar 09; Vol. 13 (1), pp. 3966. Date of Electronic Publication: 2023 Mar 09.
DOI: 10.1038/s41598-023-30907-2
Abstrakt: Optogenetics is a powerful tool for manipulating neuronal activity by light illumination with high temporal and spatial resolution. Anion-channelrhodopsins (ACRs) are light-gated anion channels that allow researchers to efficiently inhibit neuronal activity. A blue light-sensitive ACR2 has recently been used in several in vivo studies; however, the reporter mouse strain expressing ACR2 has not yet been reported. Here, we generated a new reporter mouse strain, LSL-ACR2, in which ACR2 is expressed under the control of Cre recombinase. We crossed this strain with a noradrenergic neuron-specific driver mouse (NAT-Cre) to generate NAT-ACR2 mice. We confirmed Cre-dependent expression and function of ACR2 in the targeted neurons by immunohistochemistry and electrophysiological recordings in vitro, and confirmed physiological function using an in vivo behavioral experiment. Our results show that the LSL-ACR2 mouse strain can be applied for optogenetic inhibition of targeted neurons, particularly for long-lasting continuous inhibition, upon crossing with Cre-driver mouse strains. The LSL-ACR2 strain can be used to prepare transgenic mice with homogenous expression of ACR2 in targeted neurons with a high penetration ratio, good reproducibility, and no tissue invasion.
(© 2023. The Author(s).)
Databáze: MEDLINE
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