Identification of a protein expression signature distinguishing early from organising diffuse alveolar damage in COVID-19 patients.

Autor:	Ashwin H; York Biomedical Research Institute, Hull York Medical School, University of York, York, UK., Milross L; Newcastle University Translational and Clinical Research Institute, Newcastle upon Tyne, UK., Wilson J; Department of Mathematics, University of York, York, UK., Majo J; Department of Cellular Pathology, Newcastle Upon Tyne Hospitals NHS Foundation Trust, Newcastle Upon Tyne, UK., Hang Lee JT; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Calder G; Biosciences Technology Facility, University of York, York, UK., Hunter B; Newcastle University Translational and Clinical Research Institute, Newcastle upon Tyne, UK., James S; Biosciences Technology Facility, University of York, York, UK., Lagos D; York Biomedical Research Institute, Hull York Medical School, University of York, York, UK., Signoret N; York Biomedical Research Institute, Hull York Medical School, University of York, York, UK., Filby A; Biosciences Institute, Newcastle University, Newcastle upon Tyne, UK., Bayraktar OA; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK., Fisher AJ; Newcastle University Translational and Clinical Research Institute, Newcastle upon Tyne, UK paul.kaye@york.ac.uk a.j.fisher@newcastle.ac.uk.; Institute of Transplantation, Newcastle Upon Tyne Hospitals NHS Foundation Trust, Newcastle Upon Tyne, UK., Kaye PM; York Biomedical Research Institute, Hull York Medical School, University of York, York, UK paul.kaye@york.ac.uk a.j.fisher@newcastle.ac.uk.
Jazyk:	angličtina
Zdroj:	Journal of clinical pathology [J Clin Pathol] 2023 Aug; Vol. 76 (8), pp. 561-565. Date of Electronic Publication: 2023 Mar 09.
DOI:	10.1136/jcp-2023-208771
Abstrakt:	Diffuse alveolar damage (DAD) is the histological expression of acute respiratory distress syndrome and characterises lung pathology due to infection with SARS-CoV-2, and other respiratory pathogens of clinical significance. DAD reflects a time-dependent immunopathological process, progressing from an early/exudative stage through to an organising/fibrotic stage, yet within an individual these different stages of DAD may coexist. Understanding the progression of DAD is central to the development of new therapeutics to limit progressive lung damage. Here, we applied highly multiplexed spatial protein profiling to autopsy lung tissues derived from 27 patients who died from COVID-19 and identified a protein signature (ARG1, CD127, GZMB, IDO1, Ki67, phospho-PRAS40 (T246) and VISTA) that distinguishes early DAD from late DAD with good predictive accuracy. These proteins warrant further investigation as potential regulators of DAD progression. Competing Interests: Competing interests: None declared. (© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY. Published by BMJ.)
Databáze:	MEDLINE
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