Dioclea violacea lectin increases the effect of neomycin against multidrug-resistant strains and promotes the purification of the antibiotic in immobilized lectin column.

Autor: Santos MHC; Agrarian and Environmental Sciences Center, Federal University of Maranhão, Chapadinha, MA, Brazil., Santos VF; Agrarian and Environmental Sciences Center, Federal University of Maranhão, Chapadinha, MA, Brazil., Freitas PR; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Silva RRS; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Roma RR; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Santos ALE; Agrarian and Biodiversity Sciences Center, Federal University of Cariri, Crato, CE, Brazil., Ribeiro DA; Agrarian and Biodiversity Sciences Center, Federal University of Cariri, Crato, CE, Brazil., Coutinho HDM; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Rocha BAM; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Oliveira MME; Taxonomy, Biochemistry and fungal Bioprospecting Laboratory, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil., Teixeira CS; Agrarian and Biodiversity Sciences Center, Federal University of Cariri, Crato, CE, Brazil. Electronic address: claudener@gmail.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 May 01; Vol. 236, pp. 123941. Date of Electronic Publication: 2023 Mar 07.
DOI: 10.1016/j.ijbiomac.2023.123941
Abstrakt: DVL is a Man/Glc-binding lectin from Dioclea violacea seeds that has the ability to interact with the antibiotic gentamicin. The present work aimed to evaluate whether the DVL has the ability to interact with neomycin via CRD and to examine the ability of this lectin to modulate the antibiotic effect of neomycin against multidrug-resistant strains (MDR). The hemagglutinating activity test revealed that neomycin inhibited the hemagglutinating activity of DVL with a minimum inhibitory concentration of 50 mM, indicating that the antibiotic interacts with DVL via the carbohydrate recognition domain (CRD). DVL immobilized on cyanogen bromide-activated Sepharose® 4B bound 41 % of the total neomycin applied to the column, indicating that the DVL-neomycin interaction is efficient for purification processes. Furthermore, the minimum inhibitory concentrations (MIC) obtained for DVL against all strains studied were not clinically relevant. However, when DVL was combined with neomycin, a significant increase in antibiotic activity was observed against S. aureus and P. aeruginosa. These results demonstrate the first report of lectin-neomycin interaction, indicating that immobilized DVL has the potential to isolate neomycin by affinity chromatography. Moreover, DVL increased the antibiotic activity of neomycin against MDR, suggesting that it is a potent adjuvant in the treatment of infectious diseases.
Competing Interests: Declaration of competing interest The authors declare no conflicts of interest regarding the publication of this article.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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