HPV genotypes in high-grade cervical lesions and invasive cervical carcinoma detected in Gabonese women.

Autor: Moussavou-Boundzanga P; Centre International de Recherches Médicales de Franceville (CIRMF), BP 69, Franceville, Gabon.; Laboratoire de Biologie Moléculaire et Cellulaire (LABMC), Université des Sciences et Techniques de Masuku, BP 941, Franceville, Gabon., Koumakpayi IH; Centre Hospitalier Universitaire de Libreville, Libreville, Gabon.; Institut de Cancérologie de Libreville (ICL), Libreville, Gabon., Engohan Aloghe C; Institut de Cancérologie de Libreville (ICL), Libreville, Gabon., Chansi JK; Institut de Cancérologie de Libreville (ICL), Libreville, Gabon., Revignet R; Institut de Cancérologie de Libreville (ICL), Libreville, Gabon., Leroy EM; Centre International de Recherches Médicales de Franceville (CIRMF), BP 69, Franceville, Gabon.; Institut de Recherches et de Développement (IRD), MIVEGEC, CNRS, IRD, Université de Montpellier, Montpellier, France., Berthet N; Centre International de Recherches Médicales de Franceville (CIRMF), BP 69, Franceville, Gabon. nicolas.berthet@pasteur.fr.; Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence, Institut Pasteur, Université Paris-Cité, 25 Rue du Docteur Roux, 75724, Paris, France. nicolas.berthet@pasteur.fr.; Unit of Discovery and Molecular Characterization of Pathogens, The Center for Microbes, Development and Health, Institut Pasteur of Shanghai - Chinese Academy of Sciences, Life Science Research Building, No 320 Yueyang Road, XuHui District, Shanghai, China. nicolas.berthet@pasteur.fr.
Jazyk: angličtina
Zdroj: Infectious agents and cancer [Infect Agent Cancer] 2023 Mar 08; Vol. 18 (1), pp. 16. Date of Electronic Publication: 2023 Mar 08.
DOI: 10.1186/s13027-023-00493-z
Abstrakt: Background: Cervical cancer is the third most common cancer among women worldwide, but particularly affects women living in sub-Saharan Africa. Screening and vaccination programs are two prevention approaches that can reduce cervical cancer incidence. However, effective vaccination campaigns require better knowledge of the prevalence of the main human papillomavirus (HPV) genotypes reported in high-grade neoplastic lesions and invasive carcinomas in women.
Methods: All samples collected in this study were processed using standard histopathological methods with haematoxylin and eosin staining of the sections. Areas with abnormal cells were then identified. The HPV genotype was determined on the DNA extracted from the same sections using nested PCR followed by amplicon sequencing and real-time PCR specific to five different HPV genotypes (16, 18, 33, 45 and 58).
Results: A total of 132 Gabonese patients with high-grade neoplastic lesions were included in this study; 81% were squamous cell carcinomas (SCC). At least one HPV was detected in 92.4% patients; HPV16 (75.4%) was the most frequent genotype, followed by HPV18, 58, 45, 33 and 35. Moreover, histological analysis showed that SCC samples had 50% and 58.2% stage III and IV tumor cells, respectively, according to the FIGO classification. Finally, 36.9% of these stage III and IV patients were less than 50 years old.
Conclusions: Our results confirm the high prevalence of HPV16 and 18 genotypes among high-grade lesions in Gabonese women. This study confirms the need for a national strategy for early screening of precancerous lesions associated with a broad national vaccination program among non-sexually active women to significantly reduce the long-term cancer burden.
(© 2023. The Author(s).)
Databáze: MEDLINE
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