Quantitative analyses of interactions between SpoVG and RNA/DNA.

Autor: Saylor TC; Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA., Savage CR; Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA., Krusenstjerna AC; Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA., Jusufovic N; Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA., Zückert WR; Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS, USA., Brissette CA; Department of Biomedical Sciences, University of North Dakota, School of Medicine and Health Sciences, Grand Forks, ND, USA., Motaleb M; Department of Microbiology and Immunology, East Carolina University, Greenville, NC, USA., Schlax PJ; Department of Chemistry and Biochemistry, Bates College, Lewiston, ME, USA., Stevenson B; Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA; Department of Entomology, University of Kentucky, Lexington, KY, USA. Electronic address: brian.stevenson@uky.edu.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2023 Apr 30; Vol. 654, pp. 40-46. Date of Electronic Publication: 2023 Feb 27.
DOI: 10.1016/j.bbrc.2023.02.044
Abstrakt: The Borrelia burgdorferi SpoVG protein has previously been found to be a DNA- and RNA-binding protein. To aid in the elucidation of ligand motifs, affinities for numerous RNAs, ssDNAs, and dsDNAs were measured and compared. The loci used in the study were spoVG, glpFKD, erpAB, bb0242, flaB, and ospAB, with particular focus on the untranslated 5' portion of the mRNAs. Performing binding and competition assays yielded that the 5' end of spoVG mRNA had the highest affinity while the lowest observed affinity was to the 5' end of flaB mRNA. Mutagenesis studies of spoVG RNA and ssDNA sequences suggested that the formation of SpoVG-nucleic acid complexes are not entirely dependent on either sequence or structure. Additionally, exchanging uracil for thymine in ssDNAs did not affect protein-nucleic acid complex formation.
Competing Interests: Declaration of competing interest The authors certify that they do not have any conflicts of interest with this study or report.
(Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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