Monitoring live mitochondrial metabolism in real-time using NMR spectroscopy.
Autor: | Nagana Gowda GA; Northwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.; Mitochondria and Metabolism Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA., Pascua V; Northwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.; Mitochondria and Metabolism Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA., Lusk JA; Northwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.; Mitochondria and Metabolism Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA., Hong NN; Northwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.; Mitochondria and Metabolism Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA., Guo L; Northwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA., Dong J; Northwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.; Department of Electronic Science, Xiamen University, Xiamen, China., Sweet IR; Department of Medicine, University of Washington, Seattle, Washington, USA., Raftery D; Northwest Metabolomics Research Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.; Mitochondria and Metabolism Center, Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.; Fred Hutchinson Cancer Center, Seattle, Washington, USA. |
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Jazyk: | angličtina |
Zdroj: | Magnetic resonance in chemistry : MRC [Magn Reson Chem] 2023 Dec; Vol. 61 (12), pp. 718-727. Date of Electronic Publication: 2023 Mar 23. |
DOI: | 10.1002/mrc.5341 |
Abstrakt: | Investigation of mitochondrial metabolism is gaining increased interest owing to the growing recognition of the role of mitochondria in health and numerous diseases. Studies of isolated mitochondria promise novel insights into the metabolism devoid of confounding effects from other cellular organelles such as cytoplasm. This study describes the isolation of mitochondria from mouse skeletal myoblast cells (C2C12) and the investigation of live mitochondrial metabolism in real-time using isotope tracer-based NMR spectroscopy. [3- 13 C (© 2023 John Wiley & Sons Ltd.) |
Databáze: | MEDLINE |
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