Epigenomic Profiling of Dorsal Root Ganglia upon Regenerative and Non-regenerative Axonal Injury.
Autor: | Müller F; Division of Neuroscience, Department of Brain Sciences, Imperial College London, London, UK., Chadwick JS; Division of Neuroscience, Department of Brain Sciences, Imperial College London, London, UK., Di Giovanni S; Division of Neuroscience, Department of Brain Sciences, Imperial College London, London, UK., Palmisano I; Division of Neuroscience, Department of Brain Sciences, Imperial College London, London, UK. i.palmisano@imperial.ac.uk. |
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Jazyk: | angličtina |
Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2636, pp. 101-144. |
DOI: | 10.1007/978-1-0716-3012-9_7 |
Abstrakt: | RNA sequencing (RNA-seq), chromatin immunoprecipitation sequencing (ChIP-seq), and assay for transposase-accessible chromatin sequencing (ATAC-seq) are genome-wide techniques that provide information relative to gene expression, chromatin binding sites, and chromatin accessibility, respectively. Here we describe RNA-seq, H3K9ac, H3K27ac and H3K27me3 ChIP-seq, and ATAC-seq in dorsal root ganglia (DRG) after sciatic nerve or dorsal column axotomy, to characterize the transcriptional and epigenetic signatures of DRG upon regenerative vs non-regenerative axonal lesion. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
Databáze: | MEDLINE |
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