Association Mapping Combined with Whole Genome Sequencing Data Reveals Candidate Causal Variants for Sclerotinia Stem Rot Resistance in Brassica napus .

Autor:	Newman TE; Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia., Khentry Y; Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia., Leo A; New South Wales Department of Primary Industries, Pine Gully Road, Wagga Wagga, NSW 2650, Australia., Lindbeck KD; New South Wales Department of Primary Industries, Pine Gully Road, Wagga Wagga, NSW 2650, Australia., Kamphuis LG; Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia., Derbyshire MC; Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.
Jazyk:	angličtina
Zdroj:	Phytopathology [Phytopathology] 2023 May; Vol. 113 (5), pp. 800-811. Date of Electronic Publication: 2023 Jun 23.
DOI:	10.1094/PHYTO-06-22-0217-FI
Abstrakt:	Canola ( Brassica napus ) yield can be significantly reduced by the disease sclerotinia stem rot (SSR), which is caused by Sclerotinia sclerotiorum , a necrotrophic fungal pathogen with an unusually large host range. Breeding cultivars that are physiologically resistant to SSR is desirable to enhance crop productivity. However, the development of resistant varieties has proved challenging due to the highly polygenic nature of S. sclerotiorum resistance. Here, we identified regions of the B. napus genome associated with SSR resistance using data from a previous study by association mapping. We then validated their contribution to resistance in a follow-up screen. This follow-up screen also confirmed high levels of SSR resistance in several genotypes from the previous study. Using publicly available whole genome sequencing data for a panel of 83 B. napus genotypes, we identified nonsynonymous polymorphisms linked to the SSR resistance loci. A qPCR analysis showed that two of the genes containing these polymorphisms were transcriptionally responsive to S. sclerotiorum infection. In addition, we provide evidence that homologues of three of the candidate genes contribute to resistance in the model Brassicaceae species Arabidopsis thaliana . The identification of resistant germplasm and candidate genomic loci associated with resistance are important findings that can be exploited by breeders to improve the genetic resistance of canola varieties. Competing Interests: The author(s) declare no conflict of interest.
Databáze:	MEDLINE
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