Heritable Cas9-induced nonhomologous recombination in C. elegans .

Autor:	Zdraljevic S; Department of Human Genetics, University of California, Los Angeles, CA, USA.; Department of Biological Chemistry, University of California, Los Angeles, CA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA., Walter-McNeill L; Department of Human Genetics, University of California, Los Angeles, CA, USA.; Department of Biological Chemistry, University of California, Los Angeles, CA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA., Marquez H; Department of Human Genetics, University of California, Los Angeles, CA, USA.; Department of Biological Chemistry, University of California, Los Angeles, CA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA., Kruglyak L; Department of Human Genetics, University of California, Los Angeles, CA, USA.; Department of Biological Chemistry, University of California, Los Angeles, CA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA.
Jazyk:	angličtina
Zdroj:	MicroPublication biology [MicroPubl Biol] 2023 Feb 17; Vol. 2023. Date of Electronic Publication: 2023 Feb 17 (Print Publication: 2023).
DOI:	10.17912/micropub.biology.000775
Abstrakt:	Identification of the genetic basis of phenotypic variation within species remains challenging. In species with low recombination rates, such as Caenorhabditis elegans , genomic regions linked to a phenotype of interest by genetic mapping studies are often large, making it difficult to identify the specific genes and DNA sequence variants that underlie phenotypic differences. Here, we introduce a method that enables researchers to induce heritable targeted recombination in C. elegans with Cas9. We demonstrate that high rates of targeted nonhomologous recombination can be induced by Cas9 in a genomic region in which naturally occurring meiotic recombination events are exceedingly rare. We anticipate that Cas9-induced nonhomologous recombination (CINR) will greatly facilitate high-resolution genetic mapping in this species. (Copyright: © 2023 by the authors.)
Databáze:	MEDLINE
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