Zebrafish pigment cells develop directly from persistent highly multipotent progenitors.

Autor: Subkhankulova T; Department of Life Sciences, University of Bath, Claverton Down, Bath, BA2 7AY, UK., Camargo Sosa K; Department of Life Sciences, University of Bath, Claverton Down, Bath, BA2 7AY, UK., Uroshlev LA; Vavilov Institute of General Genetics, Russian Academy of Sciences, Ul. Gubkina 3, Moscow, 119991, Russia., Nikaido M; Department of Life Sciences, University of Bath, Claverton Down, Bath, BA2 7AY, UK.; Graduate School of Science, University of Hyogo, Ako-gun, Hyogo Pref., 678-1297, Japan., Shriever N; Department of Life Sciences, University of Bath, Claverton Down, Bath, BA2 7AY, UK., Kasianov AS; Vavilov Institute of General Genetics, Russian Academy of Sciences, Ul. Gubkina 3, Moscow, 119991, Russia.; Department of Medical and Biological Physics, Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141701, Russia.; A.A. Kharkevich Institute for Information Transmission Problems (IITP), Russian Academy of Sciences, Bolshoy Karetny per. 19, build.1, Moscow, 127051, Russia., Yang X; Department of Life Sciences, University of Bath, Claverton Down, Bath, BA2 7AY, UK.; The MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, 200438, PR China., Rodrigues FSLM; Department of Life Sciences, University of Bath, Claverton Down, Bath, BA2 7AY, UK., Carney TJ; Department of Life Sciences, University of Bath, Claverton Down, Bath, BA2 7AY, UK.; Lee Kong Chian School of Medicine, Experimental Medicine Building, Yunnan Garden Campus, Nanyang Technological University, 59 Nanyang Drive, Yunnan Garden, 636921, Singapore., Bavister G; Department of Life Sciences, University of Bath, Claverton Down, Bath, BA2 7AY, UK., Schwetlick H; Department of Mathematical Sciences, University of Bath, Claverton Down, Bath, BA2 7AY, UK., Dawes JHP; Department of Mathematical Sciences, University of Bath, Claverton Down, Bath, BA2 7AY, UK., Rocco A; Department of Microbial Sciences, FHMS, University of Surrey, GU2 7XH, Guildford, UK.; Department of Physics, FEPS, University of Surrey, GU2 7XH, Guildford, UK., Makeev VJ; Vavilov Institute of General Genetics, Russian Academy of Sciences, Ul. Gubkina 3, Moscow, 119991, Russia.; Department of Medical and Biological Physics, Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141701, Russia.; Laboratory 'Regulatory Genomics', Institute of Fundamental Medicine and Biology, Kazan Federal University, 18 Kremlyovskaya street, Kazan, 420008, Russia., Kelsh RN; Department of Life Sciences, University of Bath, Claverton Down, Bath, BA2 7AY, UK. bssrnk@bath.ac.uk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Mar 06; Vol. 14 (1), pp. 1258. Date of Electronic Publication: 2023 Mar 06.
DOI: 10.1038/s41467-023-36876-4
Abstrakt: Neural crest cells are highly multipotent stem cells, but it remains unclear how their fate restriction to specific fates occurs. The direct fate restriction model hypothesises that migrating cells maintain full multipotency, whilst progressive fate restriction envisages fully multipotent cells transitioning to partially-restricted intermediates before committing to individual fates. Using zebrafish pigment cell development as a model, we show applying NanoString hybridization single cell transcriptional profiling and RNAscope in situ hybridization that neural crest cells retain broad multipotency throughout migration and even in post-migratory cells in vivo, with no evidence for partially-restricted intermediates. We find that leukocyte tyrosine kinase early expression marks a multipotent stage, with signalling driving iridophore differentiation through repression of fate-specific transcription factors for other fates. We reconcile the direct and progressive fate restriction models by proposing that pigment cell development occurs directly, but dynamically, from a highly multipotent state, consistent with our recently-proposed Cyclical Fate Restriction model.
(© 2023. The Author(s).)
Databáze: MEDLINE
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