Identification of the G184C, C190A and T2134A mutations in the para-sodium channel gene of the southern cattle tick Rhipicephalus (Boophilus) microplus associated with resistance to cypermethrin in northern Veracruz, Mexico.

Autor: Cruz-Valdés T; Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan, Universidad Veracruzana, Túxpan de Rodríguez Cano, Veracruz, Mexico., Grostieta E; Centro de Medicina Tropical, Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, Mexico., Chagoya-Fuentes JL; Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan, Universidad Veracruzana, Túxpan de Rodríguez Cano, Veracruz, Mexico., Bravo-Ramos JL; Facultad de Medicina Veterinaria y Zootecnia, Rancho Torreón del Molino, Universidad Veracruzana, Veracruz, Mexico., Ojeda-Chi M; Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan, Universidad Veracruzana, Túxpan de Rodríguez Cano, Veracruz, Mexico. Electronic address: mojeda_93@hotmail.com., Lammoglia-Villagómez MA; Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan, Universidad Veracruzana, Túxpan de Rodríguez Cano, Veracruz, Mexico., Rojas-Ronquillo R; Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan, Universidad Veracruzana, Túxpan de Rodríguez Cano, Veracruz, Mexico., Cabrera-Núñez A; Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan, Universidad Veracruzana, Túxpan de Rodríguez Cano, Veracruz, Mexico., Aguilar-Tipacamú G; C.A. Salud Animal y Microbiología Ambiental, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Querétaro, Mexico., Colunga-Salas P; Instituto de Biotecnología y Ecología Aplicada, Universidad Veracruzana, Xalapa, Veracruz, Mexico., Becker I; Centro de Medicina Tropical, Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, Mexico., Montes SS; Facultad de Ciencias Biológicas y Agropecuarias región Tuxpan, Universidad Veracruzana, Túxpan de Rodríguez Cano, Veracruz, Mexico; Centro de Medicina Tropical, Unidad de Investigación en Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. Electronic address: sok10108@gmail.com.
Jazyk: angličtina
Zdroj: Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2023 Apr; Vol. 39, pp. 100838. Date of Electronic Publication: 2023 Feb 04.
DOI: 10.1016/j.vprsr.2023.100838
Abstrakt: The southern cattle tick (Rhipicephalus microplus) represents one of the ectoparasites with the greatest distribution worldwide. Infestations by this arthropod can cause a decrease in the production of meat and milk, as well as anaemia and the transmission of bacterial and parasitic agents. For this reason, several active molecules have been developed to control these arthropods. A widely used group of ixodicides are pyrethroids, especially cypermethrin, which have knockdown effects on ticks. Resistance to cypermethrin has been reported in ticks since the 2000s; it was registered for the first time in Mexico in 2009. Even though multiple studies have evaluated resistance with conventional tests, there are few studies in Mexico that have identified the presence of single nucleotide polymorphisms (SNPs) associated with resistance. Hence, the aim of this work was to monitor three mutations associated with resistance in the sodium/chlorine channel in eight populations of ticks from northern Veracruz. Engorged adult females were collected from which genomic DNA was extracted. Subsequently, three mutations in domains II and III of para‑sodium channel gene were detected by conventional PCR and sequencing. Global alignments were done with the reference sequences deposited in GenBank. A total of 116 engorged females were analysed, of which 10 tested positive for G184C and C190A of domain II of the para‑sodium channel gene. T2134A was present in domain III in a single production unit. This is the first work where molecular monitoring of cypermethrin resistance has been carried out in the northern zone of the state of Veracruz.
Competing Interests: Declaration of Competing Interest On behalf of all the co-authors, I declare that we have no conflict of interest. Thank you for your consideration of this submission.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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