Molecular mechanisms of glycogen particle assembly in Escherichia coli.

Autor: Li F; Laboratory Medicine, The Fifth People's Hospital of Huai'an, Huai'an, Jiangsu Province, China., Wang MM; Department of Pharmacy, Qingdao Eighth People's Hospital, Qingdao, Shandong Province, China; Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu Province, China., Liu QH; State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Taipa, Macao SAR, China., Ma ZW; Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, Xuzhou, Jiangsu Province, China., Wang JJ; Department of Intelligent Medical Engineering, School of Medical Informatics and Engineering, Xuzhou Medical University, Xuzhou, Jiangsu Province, China., Wang ZY; School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia., Tang JW; Department of Intelligent Medical Engineering, School of Medical Informatics and Engineering, Xuzhou Medical University, Xuzhou, Jiangsu Province, China., Lyu JW; Laboratory Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong Province, China., Zhu ZB; Department of Genetics, School of Life Sciences, Xuzhou Medical University, Xuzhou, Jiangsu Province, China. Electronic address: zhuzuobin@xzhmu.edu.cn., Wang L; Laboratory Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong Province, China. Electronic address: wangliang@gdph.org.cn.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2023 Jan 01; Vol. 299, pp. 120200. Date of Electronic Publication: 2022 Oct 10.
DOI: 10.1016/j.carbpol.2022.120200
Abstrakt: It has been reported that glycogen in Escherichia coli has two structural states, that is, fragility and stability, which alters dynamically. However, molecular mechanisms behind the structural alterations are not fully understood. In this study, we focused on the potential roles of two important glycogen degradation enzymes, glycogen phosphorylase (glgP) and glycogen debranching enzyme (glgX), in glycogen structural alterations. The fine molecular structure of glycogen particles in Escherichia coli and three mutants (ΔglgP, ΔglgX and ΔglgP/ΔglgX) were examined, which showed that glycogen in E. coli ΔglgP and E. coli ΔglgP/ΔglgX were consistently fragile while being consistently stable in E. coli ΔglgX, indicating the dominant role of GP in glycogen structural stability control. In sum, our study concludes that glycogen phosphorylase is essential in glycogen structural stability, leading to molecular insights into structural assembly of glycogen particles in E. coli.
Competing Interests: Declaration of competing interest The authors declare no competing financial interest.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: