Gene expression profile during seed development of Bixa orellana accessions varying in bixin pigment.

Autor:	Cárdenas-Conejo Y; Laboratorio de Agrobiotecnología, Consejo Nacional de Ciencia y Tecnología (CONACYT)-Universidad de Colima, Colima, Mexico., Narváez-Zapata JA; Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Reynosa, Tamaulipas, Mexico., Carballo-Uicab VM; Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán A.C., Mérida, Yucatán, Mexico., Aguilar-Espinosa M; Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán A.C., Mérida, Yucatán, Mexico., Us-Camas R; Departamento de Estudios de Posgrado e Investigación, Instituto Tecnológico Superior de Calkiní, en el Estado de Campeche, Calkiní, Campeche, Mexico., Escobar-Turriza P; Segunda División de Biotecnología Industrial, Centro de Investigación Científica y Asistencia en Tecnología y Diseño del Estado de Jalisco, Zapopan, Jalisco, Mexico., Comai L; Plant Biology and Genome Center, University of California, Davis, Davis, CA, United States., Rivera-Madrid R; Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán A.C., Mérida, Yucatán, Mexico.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2023 Feb 15; Vol. 14, pp. 1066509. Date of Electronic Publication: 2023 Feb 15 (Print Publication: 2023).
DOI:	10.3389/fpls.2023.1066509
Abstrakt:	Diverse morphological, cellular and physiological changes occur during seed maturation in Bixa orellana when the seed tissues form specialized cell glands that produce reddish latex with high bixin amounts. Transcriptomic profiling during seed development in three B. orellana accessions (P12, N4 and N5) with contrasting morphologic characteristics showed enrichment in pathways of triterpenes, sesquiterpenes, and cuticular wax biosynthesis. WGCNA allows groups of all identified genes in six modules the module turquoise, the largest and highly correlated with the bixin content. The high number of genes in this module suggests a diversification of regulatory mechanisms for bixin accumulation with the genes belonging to isoprene, triterpenes and carotene pathways, being more highly correlated with the bixin content. Analysis of key genes of the mevalonate (MVA) and the 2C-methyl-D-erythritol-4-phosphate (MEP) pathways revealed specific activities of orthologs of BoHMGR , BoFFP , BoDXS , and BoHDR. This suggests that isoprenoid production is necessary for compounds included in the reddish latex of developing seeds. The carotenoid-related genes BoPSY2 , BoPDS1 and BoZDS displayed a high correlation with bixin production, consistent with the requirement for carotene precursors for apocarotenoid biosynthesis. The BoCCD gene member ( BoCCD4-4 ) and some BoALDH ( ALDH2B7.2 and ALDH3I1 ) and BoMET ( BoSABATH1 and BoSABATH8 ) gene members were highly correlated to bixin in the final seed development stage. This suggested a contributing role for several genes in apocarotenoid production. The results revealed high genetic complexity in the biosynthesis of reddish latex and bixin in specialized seed cell glands in different accessions of B. orellana suggesting gene expression coordination between both metabolite biosynthesis processes. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Cárdenas-Conejo, Narváez-Zapata, Carballo-Uicab, Aguilar-Espinosa, Us-Camas, Escobar-Turriza, Comai and Rivera-Madrid.)
Databáze:	MEDLINE
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