The Antioxidant Activity and Induction of Apoptotic Cell Death by Musa paradisiaca and Trigona sp. Honey Jelly in ORL115 and ORL188 Cells.
Autor: | Anuar MNN; Department of Nutrition Sciences, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, Pahang, Malaysia., Ibrahim M; Department of Nutrition Sciences, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, Pahang, Malaysia., Zakaria NH; Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia., Ichwan SJA; Dentistry Programme, PAPRSB Institute of Health Sciences, Universiti Brunei Darussalam, Gadong, Brunei Darussalam., Md Isa ML; Department of Basic Medical Sciences for Nursing, Kulliyyah of Nursing, International Islamic University Malaysia, Pahang, Malaysia., Mat Alewi NA; Department of Nutrition Sciences, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, Pahang, Malaysia., Hagar A; Department of Nutrition Sciences, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, Pahang, Malaysia., Abdul Majid FA; Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia. |
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Jazyk: | angličtina |
Zdroj: | The Malaysian journal of medical sciences : MJMS [Malays J Med Sci] 2023 Feb; Vol. 30 (1), pp. 82-91. Date of Electronic Publication: 2023 Feb 28. |
DOI: | 10.21315/mjms2023.30.1.7 |
Abstrakt: | Background: Head and neck cancer patients usually need nutritional support due to difficulties in swallowing and chewing. Therefore, this study aimed to formulate Musa paradisiaca and Trigona sp. honey jelly (MTJ) as a convenient functional food. Methods: The antioxidant properties were analysed using 2,2'-diphenyl-1 picrylhydrazyl (DPPH), ferric reducing antioxidant potential (FRAP) and 2,2'-azinodi 3-ethylbenthiazolinesulfonate (ABTS) assays. Cytotoxicity was assayed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test and the induction of apoptosis was observed via caspase-3/7 activity assay. The identification of phenolic compounds was done via ultra-high-performance-liquid chromatography coupled to mass spectrometer (UHPLC-MS/MS). Results: The antioxidant analysis exhibited: the half inhibitory concentration (IC Conclusion: The study evidenced that the antioxidant activity of MTJ could influence the induction of apoptosis in ORL115 and ORL188 in future investigations and verifications. Competing Interests: Conflict of Interest None. (© Penerbit Universiti Sains Malaysia, 2023.) |
Databáze: | MEDLINE |
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