A subfraction obtained from the venom of the tarantula Poecilotheria regalis contains inhibitor cystine knot peptides and induces relaxation of rat aorta by inhibiting L-type voltage-gated calcium channels.
| Autor: | Díaz-Peña LF; Posgrado en Ciencias Químico Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario S/N, 76010, Querétaro, Querétaro, Mexico., Daniela TO; Posgrado en Ciencias Químico Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario S/N, 76010, Querétaro, Querétaro, Mexico., Aguilar MB; Laboratorio de Neurofarmacología Marina, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Juriquilla, Querétaro, 76230, Mexico., Luis E; Cátedras CONACYT, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Circuito Exterior s/n, 04510, Ciudad de México, Mexico.; Laboratorio Nacional de Canalopatías, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad de México, Mexico., Lazcano-Pérez F; Laboratorio de Química de Biomacromoléculas, Instituto de Química, Universidad Nacional Autónoma de México, Ciudad de México, 04510, Mexico., Arreguín-Espinosa R; Laboratorio de Química de Biomacromoléculas, Instituto de Química, Universidad Nacional Autónoma de México, Ciudad de México, 04510, Mexico., Hernandez-Cruz A; Laboratorio Nacional de Canalopatías, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad de México, Mexico.; Departamento de Neurociencia Cognitiva, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad de México, Mexico., Ibarra-Alvarado C; Departamento de Investigación Química y Farmacológica de Productos Naturales, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario S/N, 76010, Querétaro, Querétaro, Mexico., García-Arredondo A; Departamento de Investigación Química y Farmacológica de Productos Naturales, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario S/N, 76010, Querétaro, Querétaro, Mexico. |
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| Jazyk: | angličtina |
| Zdroj: | Toxicon: X [Toxicon X] 2023 Feb 17; Vol. 18, pp. 100151. Date of Electronic Publication: 2023 Feb 17 (Print Publication: 2023). |
| DOI: | 10.1016/j.toxcx.2023.100151 |
| Abstrakt: | Venoms from tarantulas contain low molecular weight vasodilatory compounds whose biological action is conceived as part of the envenomation strategy due to its propagative effects. However, some properties of venom-induced vasodilation do not match those described by such compounds, suggesting that other toxins may cooperate with these ones to produce the observed biological effect. Owing to the distribution and function of voltage-gated ion channels in blood vessels, disulfide-rich peptides isolated from venoms of tarantulas could be conceived into potential vasodilatory compounds. However, only two peptides isolated from spider venoms have been investigated so far. This study describes for the first time a subfraction containing inhibitor cystine knot peptides, PrFr-I, obtained from the venom of the tarantula Poecilotheria regalis . This subfraction induced sustained vasodilation in rat aortic rings independent of vascular endothelium and endothelial ion channels. Furthermore, PrFr-I decreased calcium-induced contraction of rat aortic segments and reduced extracellular calcium influx to chromaffin cells by the blockade of L-type voltage-gated calcium channels. This mechanism was unrelated to the activation of potassium channels from vascular smooth muscle, since vasodilation was not affected in the presence of TEA, and PrFr-I did not modify the conductance of the voltage-gated potassium channel K Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Author(s).) |
| Databáze: | MEDLINE |
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