Occurrence and prevalence of antimicrobial resistance in urban karst groundwater systems based on targeted resistome analysis.

Autor: Kaiser RA; School of Environmental Studies, College of Interdisciplinary Studies, Tennessee Technological University, 1 William L Jones Drive, Cookeville, TN 38505, United States. Electronic address: rakaiser42@tntech.edu., Polk JS; Earth, Environmental, and Atmospheric Sciences Department, Ogden College of Science and Engineering, 1906 College Heights Blvd., Bowling Green, KY 42101, United States., Datta T; Department of Civil and Environmental Engineering, College of Engineering, Tennessee Technological University, 1 William L Jones Drive, Cookeville, TN 38505, United States., Keely SP; United States Environmental Protection Agency, 26 Martin Luther King Drive West, Cincinnati, OH 45220, United States., Brinkman NE; United States Environmental Protection Agency, 26 Martin Luther King Drive West, Cincinnati, OH 45220, United States., Parekh RR; Food Animal Environmental Systems Research Unit, Agricultural Research Service, United States Department of Agriculture, 2413 Nashville Road B5, Bowling Green, KY 42101, United States., Agga GE; Food Animal Environmental Systems Research Unit, Agricultural Research Service, United States Department of Agriculture, 2413 Nashville Road B5, Bowling Green, KY 42101, United States.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2023 May 20; Vol. 874, pp. 162571. Date of Electronic Publication: 2023 Mar 05.
DOI: 10.1016/j.scitotenv.2023.162571
Abstrakt: Antimicrobial resistance (AMR) is a global crisis threatening human, animal, and environmental health. The natural environment, specifically water resources, has been recognized as a reservoir and dissemination pathway for AMR; however, urban karst aquifer systems have been overlooked. This is a concern as these aquifer systems provide drinking water to about 10 % of the global population; yet, the urban influence on the resistome in these vulnerable aquifers is sparingly explored. This study used high-throughput qPCR to determine the occurrence and relative abundance of antimicrobial resistant genes (ARG) in a developing urban karst groundwater system in Bowling Green, KY. Ten sites throughout the city were sampled weekly and analyzed for 85 ARGs, as well as seven microbial source tracking (MST) genes for human and animal sources, providing a spatiotemporal understanding of the resistome in urban karst groundwater. To further understand ARGs in this environment, potential drivers (landuse, karst feature type, season, source of fecal pollution) were considered in relation to the resistome relative abundance. The MST markers highlighted a prominent human influence to the resistome in this karst setting. The concentration of targeted genes varied between the sample weeks, but all targeted ARGs were prevalent throughout the aquifer regardless of karst feature type or season, with high concentrations captured for sulfonamide (sul1), quaternary ammonium compound (qacE), and aminoglycoside (strB) antimicrobial classes. Higher prevalence and relative abundance were detected during the summer and fall seasons, as well as at the spring features. Linear discriminant analysis suggested that karst feature type had higher influence on ARGs in the aquifer compared to season and the source of fecal pollution had the least influence. These findings can contribute to the development of effective management and mitigation strategies for AMR.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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