Roles of TRPM4 in immune responses in keratinocytes and identification of a novel TRPM4-activating agent.

Autor: Otsuka Saito K; Laboratory of Advanced Cosmetic Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, Osaka, 565-0871, Japan; Fundamental Research Institute, Mandom Corp., 5-12, Juniken-Cho, Chuo-ku, Osaka, 540-8530, Japan; Laboratory of Mock Up Vaccine, Center for Vaccine and Adjuvant Research (CVAR), National Institutes of Biomedical Innovation, Health and Nutrition (NBIOHN), 7-6-8, Asagi, Saito, Ibaraki-City, Osaka, 567-0085, Japan. Electronic address: saito-ka@phs.osaka-u.ac.jp., Fujita F; Laboratory of Advanced Cosmetic Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, Osaka, 565-0871, Japan; Fundamental Research Institute, Mandom Corp., 5-12, Juniken-Cho, Chuo-ku, Osaka, 540-8530, Japan; Laboratory of Mock Up Vaccine, Center for Vaccine and Adjuvant Research (CVAR), National Institutes of Biomedical Innovation, Health and Nutrition (NBIOHN), 7-6-8, Asagi, Saito, Ibaraki-City, Osaka, 567-0085, Japan., Toriyama M; Laboratory of Advanced Cosmetic Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, Osaka, 565-0871, Japan; Laboratory of Mock Up Vaccine, Center for Vaccine and Adjuvant Research (CVAR), National Institutes of Biomedical Innovation, Health and Nutrition (NBIOHN), 7-6-8, Asagi, Saito, Ibaraki-City, Osaka, 567-0085, Japan; Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5, Takayama-Cho, Ikoma, Nara, 630-0192, Japan., Utami RA; School of Pharmacy, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung, 40132, Indonesia., Guo Z; Laboratory of Advanced Cosmetic Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, Osaka, 565-0871, Japan., Murakami M; Laboratory of Advanced Cosmetic Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, Osaka, 565-0871, Japan; Technical Development Center, Mandom Corp., 5-12, Juniken-Cho, Chuo-ku, Osaka, 540-8530, Japan., Kato H; Laboratory of Advanced Cosmetic Science, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, Osaka, 565-0871, Japan; Laboratory of Mock Up Vaccine, Center for Vaccine and Adjuvant Research (CVAR), National Institutes of Biomedical Innovation, Health and Nutrition (NBIOHN), 7-6-8, Asagi, Saito, Ibaraki-City, Osaka, 567-0085, Japan., Suzuki Y; Thermal Biology Group, Exploratory Research Center on Life and Living Systems National Institutes of Natural Sciences, 5-1, Aza-higashiyama, Myodaiji, Okazaki, Aichi, 444-8787, Japan; Division of Cell Signaling, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 5-1, Aza-higashiyama, Myodaiji, Okazaki, Aichi, 444-8787, Japan; Department of Physiological Sciences, SOKENDAI (The Graduate University for Advanced Studies), 5-1, Aza-higashiyama, Myodaiji, Okazaki, Aichi, 444-8787, Japan; Department of Physiology, Iwate Medical University, 1-1-1, Idaidori, Yahaba-cho, Shiwa-gun, Iwate, 028-3694, Japan., Okada F; Fundamental Research Institute, Mandom Corp., 5-12, Juniken-Cho, Chuo-ku, Osaka, 540-8530, Japan., Tominaga M; Thermal Biology Group, Exploratory Research Center on Life and Living Systems National Institutes of Natural Sciences, 5-1, Aza-higashiyama, Myodaiji, Okazaki, Aichi, 444-8787, Japan; Division of Cell Signaling, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 5-1, Aza-higashiyama, Myodaiji, Okazaki, Aichi, 444-8787, Japan; Department of Physiological Sciences, SOKENDAI (The Graduate University for Advanced Studies), 5-1, Aza-higashiyama, Myodaiji, Okazaki, Aichi, 444-8787, Japan., Ishii KJ; Laboratory of Mock Up Vaccine, Center for Vaccine and Adjuvant Research (CVAR), National Institutes of Biomedical Innovation, Health and Nutrition (NBIOHN), 7-6-8, Asagi, Saito, Ibaraki-City, Osaka, 567-0085, Japan; Division of Vaccine Science, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan. Electronic address: kenishii@ims.u-tokyo.ac.jp.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2023 Apr 30; Vol. 654, pp. 1-9. Date of Electronic Publication: 2023 Feb 24.
DOI: 10.1016/j.bbrc.2023.02.062
Abstrakt: The skin is a protective interface between the internal organs and environment and functions not only as a physical barrier but also as an immune organ. However, the immune system in the skin is not fully understood. A member of the thermo-sensitive transient receptor potential (TRP) channel family, TRPM4, which acts as a regulatory receptor in immune cells, was recently reported to be expressed in human skin and keratinocytes. However, the function of TRPM4 in immune responses in keratinocytes has not been investigated. In this study, we found that treatment with BTP2, a known TRPM4 agonist, reduced cytokine production induced by tumor necrosis factor (TNF) α in normal human epidermal keratinocytes and in immortalized human epidermal keratinocytes (HaCaT cells). This cytokine-reducing effect was not observed in TRPM4-deficient HaCaT cells, indicating that TRPM4 contributed to the control of cytokine production in keratinocytes. Furthermore, we identified aluminum potassium sulfate, as a new TRPM4 activating agent. Aluminum potassium sulfate reduced Ca 2+ influx by store-operated Ca 2+ entry in human TRPM4-expressing HEK293T cells. We further confirmed that aluminum potassium sulfate evoked TRPM4-mediated currents, showing direct evidence for TRPM4 activation. Moreover, treatment with aluminum potassium sulfate reduced cytokine expression induced by TNFα in HaCaT cells. Taken together, our data suggested that TRPM4 may serve as a new target for the treatment of skin inflammatory reactions by suppressing the cytokine production in keratinocytes, and aluminum potassium sulfate is a useful ingredient to prevent undesirable skin inflammation through TRPM4 activation.
Competing Interests: Declaration of competing interest The authors have no conflicts of interest to declare.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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