Gut microbiome is linked to functions of peripheral immune cells in transition cows during excessive lipolysis.

Autor: Gu F; Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.; Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou, 310058, China., Zhu S; Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.; Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou, 310058, China., Tang Y; Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.; Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou, 310058, China., Liu X; Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.; Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou, 310058, China., Jia M; Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.; Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou, 310058, China., Malmuthuge N; Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, 5403 1 Ave S, Lethbridge, AB, T1J 4B1, Canada., Valencak TG; Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.; Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou, 310058, China., McFadden JW; Department of Animal Science, Cornell University, 507 Tower Rd, Ithaca, NY, 14850, USA., Liu JX; Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.; Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou, 310058, China., Sun HZ; Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China. huizeng@zju.edu.cn.; Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou, 310058, China. huizeng@zju.edu.cn.
Jazyk: angličtina
Zdroj: Microbiome [Microbiome] 2023 Mar 03; Vol. 11 (1), pp. 40. Date of Electronic Publication: 2023 Mar 03.
DOI: 10.1186/s40168-023-01492-3
Abstrakt: Background: Postpartum dairy cows experiencing excessive lipolysis are prone to severe immunosuppression. Despite the extensive understanding of the gut microbial regulation of host immunity and metabolism, its role during excessive lipolysis in cows is largely unknown. Herein, we investigated the potential links between the gut microbiome and postpartum immunosuppression in periparturient dairy cows with excessive lipolysis using single immune cell transcriptome, 16S amplicon sequencing, metagenomics, and targeted metabolomics.
Results: The use of single-cell RNA sequencing identified 26 clusters that were annotated to 10 different immune cell types. Enrichment of functions of these clusters revealed a downregulation of functions in immune cells isolated from a cow with excessive lipolysis compared to a cow with low/normal lipolysis. The results of metagenomic sequencing and targeted metabolome analysis together revealed that secondary bile acid (SBA) biosynthesis was significantly activated in the cows with excessive lipolysis. Moreover, the relative abundance of gut Bacteroides sp. OF04 - 15BH, Paraprevotella clara, Paraprevotella xylaniphila, and Treponema sp. JC4 was mainly associated with SBA synthesis. The use of an integrated analysis showed that the reduction of plasma glycolithocholic acid and taurolithocholic acid could contribute to the immunosuppression of monocytes (CD14 + MON) during excessive lipolysis by decreasing the expression of GPBAR1.
Conclusions: Our results suggest that alterations in the gut microbiota and their functions related to SBA synthesis suppressed the functions of monocytes during excessive lipolysis in transition dairy cows. Therefore, we concluded that altered microbial SBA synthesis during excessive lipolysis could lead to postpartum immunosuppression in transition cows. Video Abstract.
(© 2023. The Author(s).)
Databáze: MEDLINE
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