Corynebacterium guaraldiae sp. nov.: a new species of Corynebacterium from human infections.

Autor: de Oliveira Sant'Anna L; Laboratory of Diphtheria and Corynebacteria of Clinical Relevance, Department of Microbiology, Immunology and Parasitology, State University of Rio de Janeiro, Rio de Janeiro, Brazil. lincolnsantanna.microuerj@gmail.com., Dos Santos LS; Laboratory of Diphtheria and Corynebacteria of Clinical Relevance, Department of Microbiology, Immunology and Parasitology, State University of Rio de Janeiro, Rio de Janeiro, Brazil., Araújo MRB; Hermes Pardini Institute, Vespasiano, Brazil., da Rocha DJPG; Department of Biotechnology, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil., Ramos JN; Laboratory of Diphtheria and Corynebacteria of Clinical Relevance, Department of Microbiology, Immunology and Parasitology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.; Interdisciplinary Laboratory of Medical Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Baio PVP; Army Chemical and Pharmaceutical Laboratory, Ministry of Defense, Brasília, Brazil., Del Peloso PF; Richet Laboratory, Rio de Janeiro, Brazil., da Costa Ferreira Leite C; Richet Laboratory, Rio de Janeiro, Brazil., Peixoto RS; Professur Fuer Mikrobiologie, Friedrich-Alexander-Universität Erlagen-Nürnberg, Erlangen, Germany., Almuzara M; Faculty of Pharmacy and Biochemistry and Bacteriology, Department of Clinical Biochemistry, University of Buenos Aires, Autonomous City of Buenos Aires, Buenos Aires, Argentina., Vay C; Faculty of Pharmacy and Biochemistry and Bacteriology, Department of Clinical Biochemistry, University of Buenos Aires, Autonomous City of Buenos Aires, Buenos Aires, Argentina., Barberis C; Faculty of Pharmacy and Biochemistry and Bacteriology, Department of Clinical Biochemistry, University of Buenos Aires, Autonomous City of Buenos Aires, Buenos Aires, Argentina., Sangal V; Faculty of Health and Life Sciences, Northumbria University, Newcastle Upon Tyne, UK., Burkovski A; Professur Fuer Mikrobiologie, Friedrich-Alexander-Universität Erlagen-Nürnberg, Erlangen, Germany., Aguiar ERGR; Virus Bioinformatics Laboratory, Center of Biotechnology and Genetics, Department of Biological Sciences, State University of Santa Cruz, Ilhéus, Brazil., Mattos-Guaraldi AL; Laboratory of Diphtheria and Corynebacteria of Clinical Relevance, Department of Microbiology, Immunology and Parasitology, State University of Rio de Janeiro, Rio de Janeiro, Brazil., Pacheco LGC; Department of Biotechnology, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil., Vieira VV; Interdisciplinary Laboratory of Medical Research, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2023 Jun; Vol. 54 (2), pp. 779-790. Date of Electronic Publication: 2023 Mar 04.
DOI: 10.1007/s42770-023-00938-y
Abstrakt: Non-diphtheria Corynebacterium species (NDC) belonging to the human skin and mucosa microbiota are frequently neglected as contaminants. However, reports of human infections by Corynebacterium spp. have increased considerably in recent years. In this study, a group of six NDC isolates of urine (n = 5) and sebaceous cyst (n = 1) from two South American countries were identified at genus level or misidentified based on API® Coryne and genetic/molecular analyses. The 16S rRNA (99.09-99.56%) and rpoB (96.18-97.14%) gene sequence similarities of the isolates were higher when compared with Corynebacterium aurimucosum DSM 44532  T . Multilocus sequence analysis (MLSA) indicated that these six NDC isolates compose a distinctive phylogenetic clade. Genome-based taxonomic analysis with the whole-genome sequences was able to separate these six isolates from other known Corynebacterium type strains. Average nucleotide identity (ANI), average amino acid identity (AAI), and digital DNA-DNA hybridization (dDDH) values between closely related type strains and the six isolates were considerably lower than the currently recommended threshold values for species circumscription. Phylogenetic and genomic taxonomy analyses indicated these microorganisms as a novel Corynebacterium species, for which we formally propose the name Corynebacterium guaraldiae sp. nov. with isolate 13 T (= CBAS 827 T  = CCBH 35012 T ) as type strain.
(© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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